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Abstract - Issue Jul 2021, 42 (4) Back
nstantaneous and historical temperature effects on a-pinene
antibacterial behavior of some essential aromatic oils against new Escherichia
coli strain of extended-spectrum Beta-lactam
H.A. Mousa2, M. Abd El-Mongy1*, M.M. Hazza3
and M.F. Awad4
Biotechnology Department, Genetic Engineering and Biotechnology Research
Institute, University of Sadat City, Sadat City, 32897, Egypt
Transfusion Services, Egypt Ministry Health and Population, Cairo
Governorate, 11516, Egypt
Department, Faculty of Science, Benha University, 13511, Egypt.
Biology, College of Science, Taif University, Taif, 21944, Saudi Arabia
*Corresponding Author Email : firstname.lastname@example.org
To evaluate the antimicrobial activities of some aromatic essential oils
against a new pathogenic Escherichia coli strain with
extended-spectrum beta-lactamase (ESBL) activity.
Methodology: A total of 66 enteric bacteria were isolated from
250 urine samples that had been collected from human patients at different
hospitals in Cairo, Egypt. Virulence factors of pathogenic isolates were
determined, and the antibacterial activities of cinnamon bark oil and clove
oil against E. coli EMH-542017 were estimated using the VITEK 2
system; this strain was confirmed as E. coli based on its 16S rRNA gene
sequence, which was deposited in NCBI GenBank. Finally, modification of the
bacterial cell wall following treatment with essential oil was observed with
transmission electron microscopy (TEM).
The results confirmed that E. coli EMH-542017 was capable of producing
hydroxamate siderophores and was resistant to 11 antibiotics, which were
significantly observed with different grades. Nevertheless, screening by the
combination disc method showed that 20 isolates were ESBL producers.
Polymerase chain reaction analysis confirmed that the selected isolate
EMH-542017 was positive for both ESBL blaCTX-M-9 genes. The addition of
cinnamon and clove oils at levels of 12.5, 25, and 50 mg ml-1 was revealed to
have high bactericidal effects on bacterial growth, and a change in bacterial
cell morphology was detected by TEM, in which the cell wall seemed to be damaged
and the flagella were lost following essential oil treatments, demonstrating
that essential oil treatments are an effective means of suppressing
multidrug-resistant E. coli.
Interpretation: Despite these results, there is an
urgent need to identify effective, safe, and economical treatments to reduce
and protect against urinary tract infections in Egyptian patients.
Key words: Cinnamon bark oil, Clove oil, Escherichia coli,
Extended-Spectrum Beta-Lactamase, Urinary tract infections
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