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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2021, 42 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Lipid and carotenoid production by Rhodosporodium toruloides ATCC 204091 using C5 and C6 sugars obtained from lignocellulosic hydrolysate


S. Sinha, G. Singh and D. Paul* 

Amity Institute of Biotechnology, Amity University, Noida- 201 313, India

*Corresponding Author Email :


Received: 02.07.2020                                                                    Revised: 27.10.2020                                                    Accepted: 08.03.2021




Aim: This study investigated the production of lipids and carotenoids and associated growth properties by the oleaginous red yeast Rhodosporodium toruloides strain ATCC 204091, using C5 and C6 sugar streams of lignocellulosic waste hydrolysate separately and in combination (C5+C6).

Methodology: Cell density, wet and dry biomass weight, concentration of total sugars and reducing sugars were determined at various time intervals during cell growth in media containing C5, C6 and mixed sugars. Lipid and carotenoids were extracted and the media were compared with respect to production.

Results: Production of lipid (22.25%) and carotenoids (19 mg l-1) was highest in C6 sugar, as compared to mixed sugars and C5 sugar.      

Interpretation: Due to the versatility of Rhodosporodium toruloides strain ATCC 204091 for utilizing C6 and C5 sugars present in waste hydrolysates, it has been projected as a good choice for cultivation in “waste” hydrolysates.

Key words: Carotenoid, hydrolysate; lignocellulosic, lipid, Rhodosporodium toruloides




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