mycobacteriosis along with concurrent fibroma in Goldfish, Carassius
auratus from India
T. R. Swaminathan1,
N. Sood2*, P. K. Pradhan2, M. K. Yadav2, R.
Kumar1, D. K. Verma2, R. Pal2, A.
Dharmaratnam1 and N. K. Sood3
Marine Fish Genetic Resources Centre, ICAR-NBFGR, CMFRI Campus, Kochi-682
Management and Exotics Division, ICAR-National Bureau of Fish Genetic Resources,
Lucknow-226 002, India
Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal
Sciences University, Ludhiana-141 012, India
*Corresponding Author Email : email@example.com
Goldfish (Carassius auratus L.), a freshwater fish belonging to the
family Cyprinidae, is an important ornamental fish species in the world.
Herewith, we report a case of systemic mycobacteriosis along with concurrent
fibroma in goldfish.
Methodology: The protruding mass and internal organs exhibiting
lesions were collected for histopathology and molecular diagnosis. The
sections of cutaneous mass were stained with haematoxylin and eosin (H&E)
and Masson trichrome, whereas sections from spleen as well as kidney were
stained with H&E and Ziehl-Neelsen. For identifying the etiological
agent, 16S rRNA gene fragment was amplified and sequenced, using genus-specific
primers for Mycobacterium sp.
In histopathology, multiple granulomas could be observed in kidney and spleen
sections. Importantly, typical acid-fast bacilli were demonstrated in
granulomas in spleen. The sequence of 16S rRNA gene fragment amplified from
spleen and kidney showed maximum similarity to Mycobacterium stomatepiae.
Besides, the histopathological characteristics of the protruding mass were consistent
with cutaneous fibroma.
Interpretation: These findings assume significance as
several Mycobacterium species, including the current one from
ornamental fish are reported to be of zoonotic significance.
Key words: Fibroma; Goldfish; Granuloma; Mycobacteriosis, 16S
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