JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
    JEB Awards

Google Search the Journal web-site:

    Abstract - Issue Jul 2021, 42 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Biomolecular characterization and efficacy evaluation of pathogen Erwinia tracheiphila against plant oils


S. Pattanayak1, G. Biswal2 and S. Das1* 

1Department of Plant Pathology, MS Swaminathan School of Agriculture, Centurion University of Technology and Management, Gajapati- 761 211, India

2Department of Plant Pathology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar-751 003, India

*Corresponding Author Email :


Received: 16.05.2020                                                                 Revised: 23.08.2020                                                   Accepted: 07.11.2020




Aim: To investigate patho-dyanamics of Erwinia tracheiphila on Cucurbita pepo with biochemical, molecular characterization and eco-friendly management approaches.

Methodology: Variability study of Erwinia tracheiphila was done by collecting the isolates from different places in Odisha. Disease severity was calculated and analyzed from 2016-18 to predict trend of emergence and disease progress. Molecular detection of pathogen was done through 16S rRNA sequencing. Biochemical characterization and identification of pathogenic nature of Erwinia tracheiphila was performed through Ortho-Nitrophenyl β-Galactoside, Esculin hydrolysis, Citrate and Malonate utilization in the presence of different carbohydrate molecules. Efficacy of eight different plant oils were tested by in-vitro inhibition zone (IVIZ) assay against Erwinia tracheiphila as eco-friendly management approaches.

Results: Analysis of disease severity data from 2016-18 ranged between 29% to 33%. Molecular detection of causal pathogen was further confirmed by 16S rRNA region sequencing with 99% identity of NCBI-GenBank published Erwinia tracheiphila (MK356446 and MK356441) database and phylogenetic analysis. The results of IVIZT revealed that the clove oil treatment showed maximum inhibitory effect on the growth of Erwinia tracheiphila followed by mustard oil and neem oil. Sesamum oil showed the lowest efficacy against Erwinia tracheiphila. Optimum temperature range between 25-28°C, 80-85% relative humidity and 150-180 mm average rainfall triggered the disease progress and turned to be a severe one under experimental agro-climatic situation.      

Interpretation: Biochemical characterization and molecular detection of causal isolate confirmed the pathogen as Erwinia tracheiphila, causing wilt disease of Cucurbita pepo. This study also revealed the potential efficacy of clove oil and mustard oil against Erwinia tracheiphila as compared to other selected plant oils.

Key words: Erwinia tracheiphila, Molecular detection, Patho-dyanamics, Plant oils



Copyright © 2021 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).