Molecular
characterization of endophytic fungal communities associated with Vitis
vinifera L. at Taif region of Saudi Arabia
S.M. Sayed1,2,*,
A.M. El-Shehawi3,4, S.A. Elarnaouty2, S.A. Al-Otaibi3,
S.A. El-Shazly3,5, S.S. Alotaibi3, R. Ibrahim6,7,
M.M. Ahmed8 and M.M. Elseehy4
1Department of
Science and Technology, University College of Ranyah, Taif University,
Taif-21974, Saudi Arabia
2Department of
Economic Entomology, Faculty of Agriculture, Cairo University, Giza-12613,
Egypt
3Department of
Biotechnology, Faculty of Science, Taif University, Taif-21974, Saudi Arabia
4Department of
Genetics, Faculty of Agriculture, Alexandria University, Alexandria-21526,
Egypt
5Department of
Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr
El-Sheikh 33511, Egypt
6Department of
Biology, Faculty of Science, Taibah University, Medina 344, Saudi Arabia
7Department of
Economic Entomology, Faculty of Agriculture, Kafrelsheikh University, Kafr
El-Sheikh 33516, Egypt
8Department of
Biochemistry, Faculty of Veterinary Medicine, University of Sadat City,
32958, Egypt
*Corresponding Author Email : samy_mahmoud@hotmail.com
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Abstract
Aim:
The current study aimed to characterize the composition of endophytic fungal
communities related to Taify grapevine leaves.
Methodology: Taify grapevine leaves were collected from three
different locations at Taif region. Total DNA was extracted and the
endophytic fungal communities were investigated using next generation
sequencing in a metagenomics approach.
Results:
The obtained data detected 26 fungal operational taxonomic units (OTUs) in
all locations with variation in most taxa among three locations. The phylum
Ascomycota dominated relative sequence abundance (90.83%), followed by
Basidiomycota at 1.82%. Other unidentified fungi at phylum level dominated
7.35% abundance level. At genus level, Alternaria and Cladosporium
genera were present at all locations, while Filobasidium and Didymella
were detected in two locations. Most genera, Penicillium,
Hanseniaspora, Fusarium, Chrysocorona and Quambalaria were
detected in one location only.
Interpretation: The present study confirmed the diversity
of endophytic fungi in Taify grapevine, including pathogenic and
non-pathogenic species. The detected species have potential to be used as
biocontrol species against economically important pathogens such as Alternaria
and Hanseniaspora. This will contribute to our understanding of
grapevine-endophytic fungi interaction and their utilization to enhance grapevine
production and quality.
Key
words:
Endophytes, Fungi, Grapevine, Metagenomic, Next generation sequencing
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