JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Mar 2021, 42 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Molecular characterization of endophytic fungal communities associated with Vitis vinifera L. at Taif region of Saudi Arabia

 

S.M. Sayed1,2,*, A.M. El-Shehawi3,4, S.A. Elarnaouty2, S.A. Al-Otaibi3, S.A. El-Shazly3,5, S.S. Alotaibi3, R. Ibrahim6,7, M.M. Ahmed8 and M.M. Elseehy4 

 

1Department of Science and Technology, University College of Ranyah, Taif University, Taif-21974, Saudi Arabia

2Department of Economic Entomology, Faculty of Agriculture, Cairo University, Giza-12613, Egypt

3Department of Biotechnology, Faculty of Science, Taif University, Taif-21974, Saudi Arabia

4Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria-21526, Egypt

5Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh 33511, Egypt

6Department of Biology, Faculty of Science, Taibah University, Medina 344, Saudi Arabia

7Department of Economic Entomology, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh 33516, Egypt

8Department of Biochemistry, Faculty of Veterinary Medicine, University of Sadat City, 32958, Egypt

*Corresponding Author Email : samy_mahmoud@hotmail.com

 

Received: 26.06.2020                                                        Revised: 07.10.2020                                                              Accepted: 28.11.2020

 

 

Abstract

Aim: The current study aimed to characterize the composition of endophytic fungal communities related to Taify grapevine leaves.

Methodology: Taify grapevine leaves were collected from three different locations at Taif region. Total DNA was extracted and the endophytic fungal communities were investigated using next generation sequencing in a metagenomics approach.

Results: The obtained data detected 26 fungal operational taxonomic units (OTUs) in all locations with variation in most taxa among three locations. The phylum Ascomycota dominated relative sequence abundance (90.83%), followed by Basidiomycota at 1.82%. Other unidentified fungi at phylum level dominated 7.35% abundance level. At genus level, Alternaria and Cladosporium genera were present at all locations, while Filobasidium and Didymella were detected in two locations. Most genera, Penicillium, Hanseniaspora, Fusarium, Chrysocorona and Quambalaria were detected in one location only.      

Interpretation: The present study confirmed the diversity of endophytic fungi in Taify grapevine, including pathogenic and non-pathogenic species. The detected species have potential to be used as biocontrol species against economically important pathogens such as Alternaria and Hanseniaspora. This will contribute to our understanding of grapevine-endophytic fungi interaction and their utilization to enhance grapevine production and quality.       

Key words: Endophytes, Fungi, Grapevine, Metagenomic, Next generation sequencing

 

 

 

Copyright © 2021 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).