Journal of Environmental Biology

Synthesis, characterization of blue fluorescent carbon nanoparticle and its in-vitro toxicity evaluation 

K.S. Kumar¹, P.K. Gayathri¹*, H. Khandelwal² and K.K. Prashanth² 

¹Department of Chemical Engineering, SSN Engineering College, Kalavakkam-603 110, India

²Department of Biotechnology, Vel Tech High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Avadi-600 062, India

*Corresponding Author Email : gayathri.kothandaraman@gmail.com

Paper received: 09.12.2019??????? ??????????? ???????????????????????????Revised received: 20.03.2020????????????? ????????????????????????????????? Accepted: 03.07.2020

Abstract

Aim: The present study aimed to synthesize and characterize carbon nanoparticles (CNPs) from a new precursor and to evaluate its effect on the viability of healthy seeds and cell lines.

Methodology: The CNPs were synthesized by acid assisted carbonization of carbohydrate and its characteristics such as size, fluorescence property and purity of samples were evaluated for confirmation. Cytotoxic studies were performed in MCF 10a cell lines using 3-4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) assay whereas the phytotoxic assay was conducted by using scallions and Indian pearl millet.

Results: Transmission electron microscope (TEM) results showed that nano-sized CNPs [10-40 nm] were synthesized and its characteristic peaks were observed in Ultraviolet (UV) and fluorescence spectrum. The Energy Dispersive X-ray (EDAX) analysis confirmed the presence of carbon and oxygen without any impurities. It was non-cytotoxic for healthy MCF 10a cell lines and did not affect the germination rate of Indian pearl millets at higher concentrations. In both toxicity studies, the viability of cells and plants was >95% even after exposure to a high concentration of CNPs.      

Interpretation: The non-cytotoxic and non-phytotoxic nature of CNPs implies that it does not affect the growth of plants, seedling germination and healthy cell lines. It can be considered as a compatible and safe material for MCF 10a cell lines and plants.       

Key words: Carbon nanoparticles, Fluorescence study, MCF 10a cell lines, MTT assay, Pearl millet

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