Abstract
Aim:
The study focused on identifying markers linked to aquaporin genes from the
expressed regions of S. melongena using bioinformatics applications.
Methodology: The EST collections were explored for identification
of aquaporin markers for water stress response using comparative analysis and
in-house developed repeat motif detection program. An algorithm was developed
to generate repeat motifs which can be effectively used for collecting EST of
S. melongena to filter the sequences having repeat motifs for further
analysis.???? ???????????????
Results:
From the results generated, the 22 potential sequences with the markers were
found to be associated with aquaporin proteins. The detected repeat motifs
are inherent part of markers and these markers are found to be evolutionarily
conserved and associated with aquaporin proteins. Hence, identifying markers
for the presence of aquaporin proteins play an important role in water
diffusion across cell membranes in plants.?? ???????????????????
Interpretation: Identifying aquaporin markers are useful
for plant breeders for developing water stress tolerant crops during elevated
temperatures. These markers are linked to water channel proteins that belong
to superfamily Major Intrinsic Protein, that primarily plays an important
role in conduction of water in plants.?? ????????
Key words: Aquaporins, Repeat motif, Solanum melongena,
Stress, Water transport
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