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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP
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    Abstract - Issue Sep 2020, 41 (5)                                     Back


nstantaneous and historical temperature effects on a-pinene

Passive transfer of immunity from Tilapia antisera enhanced in-vitro phagocytosis of Aeromonas hydrophila in na?ve Macrobrachium rosenbergii haemocytes 

 

C.M. Chong1,2*, A.S. Yaakob2 and A. Maha1,3 

1Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

2Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

3Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia

*Corresponding Author Email : choumin@upm.edu.my

 

Paper received: 18.09.2019 ?????? ???????????????????????????????????????Revised received: 26.11.2019 ???????????? ???????????????????????Accepted: 14.08.2020

 

 

Abstract

Aim: To determine the effectiveness of antisera derived from a teleost to augment the phagocytic activity of prawn phagocytes.?

Methodology: Antisera from tilapia, Oreochromis niloticus, immunised with methanol-killed Aeromonas hydrophila was added to the suspension of A. hydrophila prior to the incubation with the haemocytes isolated from Macrobrachium rosenbergii and examined for phagocytosis. The sera derived from the sterile saline-injected fish were used for comparison.

Results:? In-vitro phagocytosis assay showed that the supplementation of immunised sera significantly enhanced Aeromonas uptake by prawn haemocyte by increasing phagocytic index to 3.23 ? 0.44, compared to control with phagocytic index of 1.35 ? 0.42.

Interpretation: Tilapia antisera may potentially be adopted to provide passive immune-protection to crustaceans which lack adaptive immunity. Further studies should confirm the mechanisms involved in enhancement of prawn phagocytic uptake of bacteria by fish immunised sera.

Key words: Haemocytes, Macrobrachium rosenbergii, Passive immunity, Phagocytosis, Tilapia antisera

 

 

 

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