Destructive dose
determination of electron beam irradiation for pathogenic bacteria in water
medium by 96 well plate assay
S. Visnuvinayagam1*,
L.N. Murthy2, U. Parvathy4, A. Jeyakumari3,
K.P. Rawat5, S.A. Khadar5 and K.S.S. Sarma6
1Microbiology
Fermentation and Biotechnology Division, ICAR- CIFT, Cochin-682 029, India
2Mumbai Research
Centre of ICAR-CIFT, Vashi,? Mumbai-400 703, India
3Fish Processing
Division, Mumbai Research Centre of ICAR-CIFT, Mumbai-400 703, India
4Fish Processing
Division, ICAR- CIFT, Cochin-682 029, India
5EBPS, IRAD, BARC,
BRIT-BARC Complex, Mumbai-400 703, India
6Isotope and
Radiation Applications Division, Bhabha Atomic Research Centre, Mumbai-400
085, India
*Corresponding Author Email : visnuvinayagam@yahoo.co.in
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Abstract
Aim:
Determination of suitable dosage for eradication of different pathogenic
bacteria in water will be useful for further detailed research in this
regard.
Methodology: In the current investigation, known concentration of
different actively growing bacterial culture were inoculated in 96-well-plate
and exposed to 1, 2 and 3 kGy Electron Beam (EB) irradiation to understand
the suitable destructive dosage.
Results:
1 kGy EB irradiation completely destroyed more than 2 ? 107 number
of E. coli, approximately 2 ? 106 number of MRSA, 5
? 105 number of Salmonella, V. cholerae and S. aureus
and 1 ? 104 number of P. aeruginosa, respectively.? Based
on the study, the hierarchy of susceptible bacteria was as follows: E.
coli < MRSA < V. cholerae < S. aureus < Salmonella
< P. aeruginosa < B. cereus < L. monocytogenes.
Interpretation: The dose required for elimination of
pathogens in 96 well plate was always lesser than that required for pathogens
in the meat. 96-well-plate method is a simple and rapid method to assess
different bacteria with different concentration in a single time and, hence,
is useful and rapid technique to to determine the destructive irradiation
dose for various bacteria and chemical pollutants in the substrate.
Key
words:
electron beam, Irradiation, MRSA, Pathogens, 96-well-plate
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