Journal of Environmental Biology

Germination indices of orthodox seeds as influenced by controlled deterioration and cathodic water seed invigoration

Kayode Fatokun¹*, R.P. Beckett^2,3, Boby Varghese¹, Sershen⁴ and N.W. Pammenter¹    

¹School of Life Sciences, University of KwaZulu-Natal Westville, Durban 4000, South Africa

²School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville 3209, South Africa

³Openlab ?Biomarker?, Kazan Federal University, Kazan 420008, The Republic of Tatarstan, Russia

⁴South African Technology Network, La Lucia, Durban, 4321, South Africa

*Corresponding Author Email : kayfatokun@yahoo.com

Paper received: 15.05.2019??????? ?????????????????????????????????????? Revised received: 10.07.2019????????????? ??????????????????????????????????? Accepted: 13.08.2019

Abstract

Aim: The aim of this study was to test the effects of priming on the germination of deteriorated orthodox seeds of five plant species.

Methodology: The water content of seeds were raised to 14%, and the seeds then deteriorated to 50% viability at 40oC and 100% relative humidity. Deteriorated seeds and fresh seeds of test species were then primed with cathodic water, un-electrophorized calcium magnesium solution and deionised water. Unprimed fresh and unprimed controlled deteriorated seeds served as? controls. Germination indices were determined.??      

Results: In general, all priming treatments had positive effects on the germination indices of deteriorated seeds of all species, and a lesser extent on fresh seeds. However, seeds treated with cathodic water performed better than seeds treated with either CaMg solution or deionised water. While controlled deterioration reduced DNA concentrations and the DNA purity of seeds, priming, particularly with cathodic water had a protecting effect on DNA. Priming also boosted amylase activities in both fresh and deteriorated seeds.      

Interpretation: Results are consistent with cathodic water reducing oxidative stress during imbibition. Cathodic water seed priming, therefore, has the potential to play a significant role in the conservation of orthodox seeds.       

Key words: Cathodic water, Germination, Orthodox seeds, Pisum Sativum, Priming

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