Optimization of nutritive
factors in culture medium for the growth of hairy root and ajmalicine content
in sarpgandha, Rauwolfia serpentina
P. Bhagat1,
S.K. Verma1, S. Yadav1, A.K. Singh2, G.K.
Aseri1 and N. Khare1*
1Amity Institute of
Microbial Technology, Amity University, Jaipur - 302 015, India
2Division of
Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi -
110 012, India
*Corresponding Author Email : neerajsnkhare@gmail.com
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Abstract
Aim:
To optimize nutritive factors for hairy root development and ajmalicine content
in Rauwolfia serpentina.
Methodology: Optimization of nutritive factor was performed with
150 mg hairy root in 50 ml culture medium.Different ratio of nitrate and
ammonium ions (0:20, 10:20, 20:20, 40:20, 60:20, 20:0, 20:10, 20:40, 20:60),
different phosphate concentration (0.625 ? 5.0 mM), different culture medium
(MS, B5, ? MS, ? B5), different pH (4.0- 7.0), different concentration (2% -
4%) of carbon sources (sucrose, fructose, glucose, galactose) were evaluated
for dry weight (growth index) and ajmalicine content enhancement.
Results:
Growth of hairy root was observed best in ? MS media.Sucrose was found
significantly best among the other tested carbon sources in terms of higher
growth index (4.31) and ajmalicine content (0.0068 mg g-1d.wt.) at
3% and 3.5% sucrose concentration.NH4+ to NO3-
ratio (mM) of 40:20 and 20:10 was significantly optimum for growth index
(7.12) and ajmalicine content (0.0112 mg g-1 d. wt.). Culture
media supplemented with 0.625 mM phosphate concentration resulted into
significantly higher ajmalicine content (0.0074 mg g-1 d. wt.). However,
increase in phosphate concentration (0.625 ? 5.0 mM) resulted into continuous
enhancement of growth index. Optimum pH of 6.0 and 6.5 was observed suitable
for higher growth index (5.19) and ajmalicine content (0.0076 mg g-1d.wt.),
respectively.
Interpretation: Standardization of nutritive factor in
culture media resulted in a development of alternative strategy for large
scale production of ajmalicine, a pharmaceutically important alkaloid.
Key
words:
Ajmalicine, Growth index, Hairy root culture, Rauwolfia serpentina
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