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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Sep 2020, 41 (5)                                     Back

nstantaneous and historical temperature effects on a-pinene

Optimization of nutritive factors in culture medium for the growth of hairy root and ajmalicine content in sarpgandha, Rauwolfia serpentina 


P. Bhagat1, S.K. Verma1, S. Yadav1, A.K. Singh2, G.K. Aseri1 and N. Khare1

1Amity Institute of Microbial Technology, Amity University, Jaipur - 302 015, India

2Division of Genomic Resources, National Bureau of Plant Genetic Resources, New Delhi - 110 012, India

*Corresponding Author Email :

Paper received: 31.10.2019 ?????? ???????????????????????????????????????Revised received: 08.01.2020 ???????????? ???????????????????????????????????Accepted: 01.04.2020



Aim: To optimize nutritive factors for hairy root development and ajmalicine content in Rauwolfia serpentina.

Methodology: Optimization of nutritive factor was performed with 150 mg hairy root in 50 ml culture medium.Different ratio of nitrate and ammonium ions (0:20, 10:20, 20:20, 40:20, 60:20, 20:0, 20:10, 20:40, 20:60), different phosphate concentration (0.625 ? 5.0 mM), different culture medium (MS, B5, ? MS, ? B5), different pH (4.0- 7.0), different concentration (2% - 4%) of carbon sources (sucrose, fructose, glucose, galactose) were evaluated for dry weight (growth index) and ajmalicine content enhancement.

Results: Growth of hairy root was observed best in ? MS media.Sucrose was found significantly best among the other tested carbon sources in terms of higher growth index (4.31) and ajmalicine content (0.0068 mg g-1d.wt.) at 3% and 3.5% sucrose concentration.NH4+ to NO3- ratio (mM) of 40:20 and 20:10 was significantly optimum for growth index (7.12) and ajmalicine content (0.0112 mg g-1 d. wt.). Culture media supplemented with 0.625 mM phosphate concentration resulted into significantly higher ajmalicine content (0.0074 mg g-1 d. wt.). However, increase in phosphate concentration (0.625 ? 5.0 mM) resulted into continuous enhancement of growth index. Optimum pH of 6.0 and 6.5 was observed suitable for higher growth index (5.19) and ajmalicine content (0.0076 mg g-1d.wt.), respectively.      

Interpretation: Standardization of nutritive factor in culture media resulted in a development of alternative strategy for large scale production of ajmalicine, a pharmaceutically important alkaloid.       

Key words: Ajmalicine, Growth index, Hairy root culture, Rauwolfia serpentina



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