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Abstract - Issue Jul 2020, 41 (4) Back
nstantaneous and historical temperature effects on a-pinene
A preliminary study
on bacterial composition in the subgingival plaque of woman with
periodontitis during pregnancy and menopause
K.I. Han1,2,
Y.R. Seo1, B.B. Patnaik3, 4, H.J. Kwon1,
E.G. Jung1, K.W. Nam1, W.J. Kim1, J.S. Lee2
and M.D. Han1*
1Department of Life
Science and Biotechnology, College of Natural Sciences, Soonchunhyang
University, Chungnam, 31538, Republic of Korea
2Korean Collection
for Type Cultures, Korea Research Institute of Bioscience and Biotechnology
(KRIBB), Jeollabuk-do, 56212, Republic of Korea
3Department of
Biotechnology, Trident Academy of Technology, Bhubaneswar-751 024, India
4PG Department of
Biosciences and Biotechnology, Fakir Mohan University, Balasore- 756 089,
India
*Corresponding Author Email : mdhan@sch.ac.kr
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Paper
received: 13.01.2020 ?????? ???????????????????????????????????????Revised
received: 09.03.2020 ???????????? ?????????????????????????????????????????Accepted:
04.04.2020
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Abstract
Aim:
The aim of the present study was to explore the bacterial composition in
subgingival plaque of females with periodontitis during pregnancy and
menopause stages using 16S ribosomal RNA (rRNA) gene pyrosequencing approach.
Methodology: Subgingival plaque was collected from four woman
volunteers (healthy, periodontitis, periodontitis at pregnancy and
periodontitis at menopause). The microbial community composition was analyzed
by 454/Roche GS FLX chemistry pyrosequencing approach using the variable
(V1-V3) region of the 16S rRNA gene. Pyrosequencing reads were sorted to get
the clean reads that were annotated against the EzBioCloud data base for
taxonomic classification. Operational Taxonomic Units (OTUs) were assigned
and shared, and subsequently identified using CLCOMMUNITY software.
Results:
Pyrosequencing yielded 13,939 sequences comprising of 13 phyla, 124 genera,
and 372 species. The predominant microbial phyla in subgingival plaque of all
woman volunteers included Firmicutes, Actinobacteria, Fusobacteria,
Bacteroidetes, and Proteobacteria. In the healthy volunteer, Streptococcus
(52.4%) formed the predominant genus while in woman with periodontitis Streptococcus
(24.6%) and Fusobacterium (11.7%) predominated. In the periodontitis
volunteer with pregnancy, the predominant genus included Streptococcus
(25.8%) and Fusobacterium (22.4%), whereas volunteer with menopause,
the gingivitis was associated with genus Alloprevotella (19.5%), Leptotrichia
(14.3%), Fusobacterium (12.3%), and Porphyromonas (12.0%).
Interpretation: This study proves on preliminary basis
that the subgingival microbiome of woman with periodontitis at pregnancy or
menopause tend to differ from that of healthy woman, and these species
included certain periodontal pathogens such as Fusobacterium nucleatum
and Porphyromonas gingivalis.
Key words: 16S rRNA gene,
Metagenomics, Oral microbiome, Periodontitis, Subgingival plaque
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