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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2020, 41 (4)                                     Back

nstantaneous and historical temperature effects on a-pinene

Effect of nitrate, ammonium and phosphate on the growth and microcystin production of Korean Microcystis species


J.H. Lee1, K.L. Lee2, J.Y. Lee3 and? H.S. Kim1*

1Department of Biology, Kyungpook National University, Daegu, 41566, Republic of Korea

2National Institute of Environmental Human Resources Development, Incheon,22689, Republic of Korea

3National Institute of Environmental Research, Incheon, 22689, Republic of Korea

*Corresponding Author Email :

Paper received: 04.10.2019 ?????? ???????????????????????????????????????Revised received: 03.02.2020 ???????????? ???????????????????????????????Accepted: 11.04.2020



Aim: This study aimed to investigate the effects of nitrogen (NO3-N, NH3-N) and phosphorus (PO4-P) on the growth and microcystin production of two bloom-forming Microcystis species (toxic M. aeruginosa MAHC160824 and non-toxic M. viridis MVHC160824).

Methodology: The two Microcystis species were isolated from the lower reaches of the Nakdong river, South Korea. In the culture experiments, the average nutrient concentrations (NH3-N, NO3-N and PO4-P) at which Microcystis appeared (> 15?C) was used as control medium. Different concentrations of NH3-N, NO3-N and PO4-P were then employed in nutrient testing (control, vs. 4 times and 16 times higher than the control). Microcystin levels were measured using a UPLC? (LC MS/MS) system.            

Results: Both toxic and non-toxic Microcystis strains exhibited a maximum cell density at 30?C and a maximum growth rate at 25-30?C. In the nutrient addition assays, the maximum growth of two Microcystis species were found at nutrient concentrations 4 to 16 times higher than the control (NH3-N: 0.468 mg l-1, PO4-P: 0.100 mg l-1, NO3-N: 32.5 mg l-1). The highest microcystin production levels were found under optimal growth conditions. The microcystin levels of toxic M. aeruginosa MAHC160824 were below the detection limit despite a higher number of cells (> 300,000 cells ml-1) at the same nutrients concentrations as those found in raw water from the Nakdong river.? ?????      

Interpretation: Higher production of microcystin occurs when there is an increase in NH3-N and PO4-P within a restricted range in toxic species M. aeruginosa MAHC160824, else the production is low.  

Key words: Microcystins, Microcystis species, Nakdong river, Nitrogen, Phosphorus




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