Journal of Environmental Biology

Exploration of new effective fibrinolytic agent from Alcaligenes aquatilis PJS_1 from polluted soil

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N. Prabhu¹, K. Sangeetha¹, C. Jayanthi¹ and T. Gajendran²* 

¹Department of Biotechnology, Vivekanandha College of Engineering for Women, Tiruchengode-637 205, India

²Department of Biotechnology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli-620 024, India

*Corresponding Author Email : gajendran01@gmail.com

Paper received: 21.02.2019??????? ??????????????? ???????????????????????Revised received: 25.07.2019????????????? ????????????????????????????? Accepted: 25.10.2019

Abstract

Aim: To isolate and identify Alcaligenes aquatilis PJS_1? from slaughter house soil samples for production of enzymatic fibrinolytic agent production

Methodology: Fibrinolytic enzyme producing bacterium was isolated from slaughter house soil samples and identified by biochemical tests and 16S rRNA sequencing. The fibrinolytic enzyme production media was optimized by various factors like energy sources, pH and temperature. Bioreactor used in the experiment was designed with suitable parameters for effective production and purification is by gel filtration chromatography. Blood clotting assay was performed to determine its anticoagulant property.?? ???????

Results: The isolated enzyme producing bacterium was identified as Alcaligenes aquatilis PJS_1. The medium with fructose and urea at pH 7.0 was found to have optimum production when incubated for 24 hr at 37?C. The crude enzyme was purified by acetone precipitation followed by gel filtration chromatography. The enzyme showed a final specific activity of 629.32 Umg^-1 with of 88.24% yield??????

Interpretation: The present study provides information that the enzyme produced by Alcaligenes aquatilis PJS_1 acts as an effective fibrinolytic agent ???

Key words: Alcaligenes sp., Anticoagulant, Bioreactor, Fibrinolytic enzyme

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