JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue May 2020, 41 (3)                                     Back


nstantaneous and historical temperature effects on a-pinene

Characterization of a novel antifungal protein from Acacia senegal seeds

                                                                                                                                             

D.S. Solanki1, S. Kumar1, K. Parihar1, A. Tak1, P. Gehlot1*, R. Pathak2 and S.K. Singh2 

1Department of Botany, Jai Narain Vyas University, Jodhpur-342 001, India

2Division of Plant Improvement and Pest Management, ICAR-Central Arid Zone Research Institute, Jodhpur-342 003, India

*Corresponding Author Email : drpg73@rediffmail.com

Paper received: 03.09.2019??????? ?????????????????????????????????????? Revised received: 23.11.2019? ??????????? ?????????????????????????????? Accepted: 26.11.2019

 

Abstract

Aim: The aim of the present study was to isolate antifungal protein from seeds of Acacia senegal in order to develop a new, effective and environmental friendly biofungicide.

Methodology: Antifungal protein from A. senegal seeds was extracted and purified through ammonium sulphate precipitation, dialysis, ion exchange and gel filtration chromatography. The novel antifungal was characterized employing SDS-PAGE, chitinase activity and antifungal efficacy. The purified protein was also characterized through MALDI-TOF MS/MS.          

Results: The yield of purified antifungal protein was estimated to be 0.96 mg 25 g-1 seeds and its molecular mass determined by SDS PAGE was 52.9 kDa. The purified protein exhibited antifungal activity against phytopathogenic fungi viz., Macrophomina phaseolina and Fusarium oxysporum also possessed chitinase activity. The purified protein was characterized through MALDI-TOF MS/MS and its spectra revealed 14 peptides with their specific amino acid sequences.??????? ???     

Interpretation: The antifungal protein isolated from A. senegal seeds has broad-spectrum antifungal activity with chitinase activity against pathogenic fungi that can be exploited for management of fungal disease as biopesticide to promote sustainable agriculture.? ?????   

Key words: Acacia senegal, Antifungal protein, Chitinase activity, MALDI TOF-Mass spectroscopy, Phytopathogenic fungi

 

 

Copyright ? 2020 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).