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Abstract - Issue Mar 2020, 41 (2) Back
nstantaneous and historical temperature effects on a-pinene
Agro-food waste employed
to design and develop culture media for fungal growth
A. I.
Grandes-Blanco1, A. L. Antonio-Flores2, R.
Garc?a-Barrientos2, S. Tlecuitl-Beristain2, G.
D?az-God?nez3 and
L.
S?nchez-Minutti2*
1Laboratory of
Research in Chemical Nutrition, Autonomous University of Tlaxcala,? 90930,
Tlaxcala, M?xico
2Biotechnological
Processes Laboratory,? Polytechnical University of? Tlaxcala, 90180,
Tlaxcala, M?xico
3Laboratory of
Biotechnology, Research Center for Biological Sciences, Autonomous University
of Tlaxcala, 90000, Tlaxcala, M?xico
*Corresponding Author Email : minutti85@hotmail.com
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Paper
received: 03.09.2019??????? ?????????????????????????????????????? Revised
received: 03.10.2019????????????? ?????????? Accepted: 26.11.2019
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Abstract
Aim:
Waste from the agricultural and food industries could be used as a source of
nutrients in the culture media for fungal growth and thus reduce
environmental pollution and the cost of bioprocesses. In this work, it was
determined if some agro-food waste can improve the mycelial growth of fungi
and laccase production.
Methodology: Culture media with agar and vermicompost, vermiwash,
calcined pork bones or fish waste for mycelial growth of fungi were
elaborated. The laccase activity of each strain grown in all culture media
was also estimated.?????
Results:
Pleurotus eryngii, Lentinula edodes, Pleurotus ostreatus, Flammulina
velutipes, Pleurotus citrinopileatus and Ganoderma lucidum grew in all
culture media, except Lentinula edodes, obtaining in general higher
biomass and radial growth rate than those observed on potato-dextrose agar.
The maximum laccase activity was found in Pleurotus citrinopileatus
cultivated in fish waste medium. The C: N ratio, ashes and pH were important
factors for the growth of the strains.??????
Interpretation: There were significant differences in
biomass concentration and laccase activity in the designed culture media.
These results provide an interesting alternative for the use of wastes and
metabolites production of industrial interest.??
Key words: Agro-waste,
Calcined bones, Fungi, Laccase activity, Vermicompost
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