Journal of Environmental Biology

Biochemical and antioxidant alterations in fresh water bivalve, Lamellidens marginalis exposed to malachite green dye

Paper received: 09.08.2018??????? Revised received: 01.12.2018???? Re-revised received: 20.01.2019 Accepted: 01.05.2019

Authors Info

R.S. Khandekar and

D.V. Muley*

Department of Zoology,

Shivaji University,

Kolhapur? 416 004, India

*Corresponding Author Email :

drdvmuley@gmail.com

Abstract

Aim: To determine acute toxicity (96 hrs) of malachite green for Lamellidens marginalis and to assess it?s biochemical consequences and antioxidant response against control.      

Methodology: Fresh water bivalve, Lamellidens marginalis were collected from Rajaram tank, Kolhapur and acclimatized for 7 days. To ascertain the acute effect (96 hrs) of malachite green dye, static bioassay was conducted at 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2 ppm on fresh water bivalve, Lamellidens marginalis. The rate of mortality was recorded and LC₀ and LC₅₀ values for 96 hrs were calculated. Estimation of biomolecules like protein, glycogen and lipid and antioxidant activity in different tissues of the organism was estimated.???     

Results: The LC₀ and LC₅₀ values were determined by static bioassay method. The effects of LC₀ and LC₅₀ concentrations of MG were evaluated by examining the biochemical profile and antioxidant response against control in different tissues like gill, mantle, hepatopancreas and gonad. The maximum significant decrease in glycogen (-29%) (-40.86) and protein (-31.73%) (-43.63%) content was observed in gills at both the concentrations LC₀ and LC₅₀ respectively as compared to other tissues. There was highly significant depletion in protein content of all the tissues. Lipid content in all tissues at LC₀ concentration showed moderately significant depletion (p <0.01), while at LC₅₀ concentration highly significant depletion (p< 0.001) was recorded. From SOD, CAT and GPx enzymes, SOD enzyme activity increased significantly (p < 0.001) in all tissues at LC₅₀ concentration. Gill and heptopancreas showed significant increase in antioxidant enzymes response at both, LC₀ and LC₅₀ groups. ? 

Interpretation: Malachite dye induces toxicity, by lowering biochemical content and inducing antioxidant enzyme activity.

Key words: Antioxidant enzymes, Hepatopancreas, Lamellidens marginalis, Malachite green, Static bioassay

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