D.S. Kim, Y.H. Yu, K.H. Lee,
J.H. Kang, D.Y. Yoo, K.H. Park and
Department of Anatomy, Soonchunhyang
University, Cheonan, 31151, Republic of Korea
Author Email :
Aim: In the present
study, we investigated the expression of nitric oxide synthase in the rabbit
sublingual glands and evaluated the effects of N ω- Nitro-L-arginine
methyl ester hydrochloride (L-NAME) on NOS expression.
Methodology: In order to
identify the morphological characteristics of sublingual glands, histological
analysis of HE staining was performed. Immunohistochemistry using monoclonal
mouse anti-endothelial NOS and anti-neuronal NOS was performed oneday and
three days after L-NAME treatment in the salivary glands of rabbits.
Results: Endothelial NOS
(eNOS) immunoreactivity was observed in the secretory duct system of
sublingual gland of rabbit. L-NAME administration significantly decreasede
NOS-positive immunoreactivities, but restored three days after L-NAME
administration. Neuronal NOS (nNOS)-positive immunoreactivity was detected in
the nerve fibers, however, unlike eNOSexpression, L-NAME administration did
not significantly decrease nNOS immunoreactivity one day after treatment.
Interpretation: The present study
suggests that endogenous NOS is certainly present in sublingual glands of
rabbits, and that the presence of eNOS and nNOS in the sublingual glands may
be closely related with the actions of NO on regulating salivary secretion.
Key words: Immunoreactivity, N ω- Nitro-L-arginine methyl ester
hydrochloride, Nitric oxide synthase, Sublingual gland