Journal of Environmental Biology

Enhancement of α-amylase production in pelleted Aspergillus tamarii through optimization for desizing of cotton fabric

Paper received: 13.11.2018??????? ??????????????????? Revised received: 12.01.2019???????? ??????????????????? Re-revised received: 22.01.2019???? ??????????????? Accepted: 28.01.2019

Authors Info

R. Mohan¹, R. Subramanian²,???? S. Muthiah³ and S. Natarajan¹* ?

¹Department of Textile Technology, Anna University, Chennai?600 025, India

²Center for Biotechnology,? Anna University, Chennai?600 025, India

³Biological Material Laboratory, CSIR-Central Leather Research Institute, Chennai?600 020, India

*Corresponding Author Email :

nselvaauc@gmail.com

Abstract

Aim: The present study was carried out to enhance the production of α-amylase by pelleted Aspergillus tamarii through optimization of various media compositions and cultivation conditions using shake flask and bioreactor experiments. ??

Methodology: Shake flask experiments were conducted to study the effect of pH, temperature and starch concentration using Response Surface Methodology (RSM) and other parameters, namely, nitrogen source, inoculum size and incubation days using single variable optimization technique for the pelleted growth of microorganism and amylase production. Scale up study was carried out for the assessment of results obtained from shake flask experiments using a laboratory scale bioreactor. In the bioreactor study, parameters, namely, pH control, agitation and aeration were considered.

Results: Maximum amylase production using pelleted Aspergillus tamarii was achieved at initial pH 6.7, temperature 30.5 ?C, 0.5% w/v starch, 0.1% w/v urea, 1.5% v/v inoculum size and 4 days of fermentation in the shake flask experiments. Filamentous growth was observed when the concentration of starch used was above 2%. The specific enzyme activity increased to 2.77 fold after partial purification. When enzyme was used for desizing cotton fabric, it produced 90% efficiency. The scale-up experiments revealed initial pH 6.7, agitation 300 rpm and aeration 1 vvm as the conditions suitable for pelleted growth, as well as to achieve maximum amylase production.? ???    

Interpretation: The results indicate pelleted growth of Aspergillus tamarii and in turn achievement of maximum amylase activity depends on media composition and fermentation conditions used at the time of enzyme production. Efficient desizing of cotton fabric by amylase showed its effectiveness towards hydrolysis of starch and converting it to soluble products for easy removal.

Key words: Aspergillus tamarii, Cotton fabric, Desizing, Optimization, α-amylase

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