R. Gnanasekaran, J. Iyyappan*, S.
Deepeka, R. Mageshwari,
S. Saranraj and
Department of Biotechnology, Vel Tech
High Tech Dr. Rangarajan Dr. Sakunthala Engineering College, Chennai ? 600
Author Email :
Aim: Hydrocarbon of
crude oil and other oil products such as gasoline comprise aromatic,
aliphatic and alicylic components pollute water bodies are hazardous. Microbial
degradation of these oil spills are an efficient way. Degrading oil
contamination by microorganism is the theme of this paper. To study degrading
efficiency of crude oil hydrocarbon by fungi.
Methodology: Fungal species
were isolated from crude oil soaked soil samples and oil contaminated soil
samples from commercial oil industry. Isolated fungal species were cultured
on nutrient media such as potato dextrose agar (PDA) and czapekdox agar at 37
?C for 7 day. Further, fungal species were identified by 18 S rRNA sequencing.
The potential of fungi to degrade hydrocarbon was investigated through
laccase and peroxidase. To examine the ability to degrade oil, the fungal
species were screened on Czapekdox agar and broth medium containing petroleum
derivatives. Laccase activity was performed by ABTS (2, 2̍-azinobis
3-ethyl-benzothiazoline-6-sulphonate) and bromophenol. They were used as
substrate for screening laccase activity.
Results: Among the
isolated eight fungal species, isolate FGS -4 showed better biodegradation
ability and subsequent 18 S rRNA revealed that isolate FGS-4 belongs to Aspergillus
tubigensis. Production of laccase enzyme was confirmed. The results of
gas chromatography analysis showed that hydrocarbon was biodegraded by the
isolated Aspergillus tubigensis.
Interpretation: Biodegradation of
hydrocarbon by Aspergillus species would create an impact on various waste
treatment methods. Eco-friendly degradation of hydrocarbons could be achieved
by Aspergillus species.
Key words: Bioremediation, Fungal species, Hydrocarbons, Laccase
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