Journal of Environmental Biology

 DOI : http://doi.org/10.22438/jeb/40/3(SI)/Sp-10

Identification of yeasts isolated from Chinese light cream in Inner Mongolia

Paper received: 17. 04. 2018?????????????????????????????????????????????????????? Revised received: 14. 11.2018??????????????????????????????????????????????????????????????????????????? Accepted: 03.01. 2019

Authors Info

W. Ye^1,2,3*, J. Chen², G. Huo²,

P. Du², A. Li² and L. Yang²     

¹College of pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai, 200 235, China

²Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University,

Harbin, 150 030, China

³Health School Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 200 235, China

*Corresponding Author Email :

48472732@99.com 

Abstract

Aim: This study was carried out to identify twenty-one yeast strains isolated from Chinese light cream in Inner Mongolia.    

Methodology: The morphological, physico-chemical characterization of yeast strains were performed. ID 32 C system was used to examine the carbohydrate fermentation. The 26S rDNA sequencing of yeasts were amplified by the primers (26SrDNA1: 5' GCA TAT CAA TAA GCG GAG GAA AAG 3'; 26SrDNA2: 5' GGT CCG TGT TTC AAG ACG G 3').

Results: The phylogenetic tree was constructed. According to the results of BLAST in NCBI, twenty-one yeasts were identified as Kluyveromyces marxianus (9), Pichia kudriavzevii (8), Saccharomyces cerevisiae (3) and Kluyveromyces lactis (1) according to 26S rDNA representative amplicons sequencing. Kluyveromyces marxianus and Pichia kudriavzevii were the main yeast strains present in light cream. ?

Interpretation: The Chinese light cream is a traditional milk product popular in Mongolia. Some researchers pay more attention on the lactic acid bacteria, but few reports on the yeast in it. In traditional fermentation method, yeast is also contributed to the flavor of light cream. So we identified the yeast of it.

Key words: 26S rDNA, Light cream, Inner Mongolia, Yeast

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