P.K. ?evik1, A.B.
Eroğlu2,? G. Yildizli2, D. Coşan1,
?. Kantar3 and G. Coral2*?
Biology, Faculty of Science and Letters, Mersin University, Mersin, 33343,
Biotechnology, Faculty of Science and Letters, Mersin University, Mersin,
Environmental Engineering, Faculty of Engineering, ?anakkale Onsekiz Mart
University, ?anakkale, 17100, Turkey
Author Email :
Aim: The aim of this
study was to isolate, identify and characterize diethyl phthalate degrading
bacterial strains isolated from a crude oil contaminated soil from a landfill
dump site of a petroleum refinery in Mersin, Turkey.
Methodology: The bacteria was
isolated from a crude oil contaminated soil and characterized by 16S rRNA
analysis. Bacterial genomic DNA was identified by 16S rRNA analyses.
Biodegredation experiments were conducted for 5 days and plasmid curing
experiment was performed. Catechol test was carried out to determine
phthalate degradation pathways.
Results: The isolated
bacteria from soil were identified as Pseudomonas putida based on 16S
rRNA sequences. The size of the plasmid was estimated to be about 15.9 kb.
Results of biodegradation experiments indicated that the diethyl phthalate
concentrations were reduced by 85.5% after 5 days of incubation at pH 7.0 and
30?C. The ability of P. putida degrading diethyl phthalate
was found to be plasmid-mediated through curing experiments.
Interpretation: The study
suggested that plasmid of Pseudomonas putida PAG5 could be involved in
effective degradation of diethyl phthalate
Key words: Biodegradation, Diethyl phthalate, Phthalate esters,
Plasmid, Pseudomonas putida