J.T. Joseph1, R.
K. Revathy1, K.
Panneerselvam2, P. Manikandan3,4 and
Microbiology,Dr. G.R. Damodaran College of Science, Coimbatore – 641 014,
Biotechnology, Dr. G.R. Damodaran College of Science, Coimbatore – 641 014,
Microbiology, MR. Government Arts College, Mannargudi – 614 014, India
Hospital and Postgraduate Institute of Ophthalmology, Coimbatore, Tamilnadu–
641 014, India
Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah
University, Kingdom of Saudi Arabia - 11952
Microbiology, PSG College of Arts & Science, Coimbatore
– 641 014, India
Author Email :
Aim: This study aims to
investigate the ability of laccase producing fungal strains Cladosporium
uredinicola GRDBF21 and Bipolaris maydis GRDBF23 isolated from
decaying wood bark in decolouration and detoxification of tannery effluent.
Methodology: Fungal strains
from decaying wood bark samples were isolated by serial dilution technique
followed by single spore isolation method. The selected fungal isolates were
investigated for their laccase enzyme production. Their effect on
physio-chemical properties of tannery effluent collected from final effluent
drainage of a leather-tanning factory in Chrompet, Chennai, Tamil Nadu, India
was analysed. Toxicity of treated and untreated tannery effluent was analysed
by seed germination test.
Results: The lignolytic
and constitutive producers of laccase enzyme, C. uredinicola GRDBF21
and B. maydis GRDBF23 exhibited a tolerance index of 1.2 and 1.5,
respectively, at 60% effluent concentration. The isolates were able to
increase pH and reduce colour, turbidity, total suspended solids and
electrical conductivity of the effluent. Besides observing a decrease in the
BOD and COD levels, there was also a reduction in the sodium and hexavalent
chromium content. C. uredinicola GRDBF21 and B. maydis GRDBF23
treated effluent showed a seed germination percentage of 66.6% and 76.6%,
respectively. The untreated effluent completely inhibited the seed
Interpretation: The study
confirms that the fungal species C. uredinicola GRDBF21 and B.
maydis GRDBF23 could be effectively used in decolouration and
detoxification of tannery effluent.
Key words: Ascomycetes fungi, Bioremediation, Laccase, Lignolytic
enzymes, Tannery effluent