B. Sowmiya and
Biotechnology, Anna University, Chennai- 600 025, India
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Aim: The current study
aimed to evaluate the potential of milk whey medium to produce low value
plantarum NZ7100 was obtained from NIZO Food Research, Netherlands. To
analyze optimal biomass, desired product and precursor production under
whey/whey permeate supplemented with various yeast extract concentration (0,
5, 10, 15 and 20 g l-1) and other economical media, batch
fermentation was conducted and metabolites were analyzed using HPLC.
Results: The study showed
that whey permeate containing 15 g l-1 yeast extract supported
maximum biomass formation of 1.7 g l-1 with significant production of total
lactic acid and D-lactic acid of about 9.78 g l-1 and 4.41 g l-1.
The kinetic parameters evaluated with commercial growth medium such as
MRS-glucose and MRS-lactose demonstrated relatively lesser growth and lactate
yield in whey permeate medium than in MRS medium.
Interpretation: The study
demonstrated the prospective of utilizing whey permeate medium to co-produce
effective, eco- friendly, low cost natural preservatives using L.
plantarum for usage in food and feed industry.
Key words: Growth kinetics, Lactic acid, Lactobacillus plantarum,
Whey permeate, Yeast extract
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