Journal of Environmental Biology

Biological characteristics and lytic effectiveness of phages isolated from domestic wastewater against indigenous Salmonella spp.

Paper received: 13.12.2017                    Revised received: 23.03.2018                      Re-revised received: 25.07.2018                      Accepted: 13.08.2018

Authors Info

S. Budiarti¹*, R.N. Sunarti², 

S.A.P. Listya¹ and I. Rusmana¹ 

¹Department of Biology, Faculty of Mathematics and Natural Science,

Bogor Agricultural University, Bogor,

16680, West Java, Indonesia

²Faculty of Science and Technology,

State Islamic University of Raden Fatah, Palembang, 30151, Indonesia

*Corresponding Author Email :

s_budiarti@yahoo.com

Abstract

Aim: This study was carried out to isolate and study the effectiveness of lytic phage from domestic wastewater to reduce the population of Salmonella spp. in patients suffering from diarrhea and to characterize biological phages.  

Methodology: The lytic phages from several domestic wastewater were identified using a transmission electron microscope to know morphological phages. After identifying the molecular weight protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, to know the effectiveness, the lytic phages were infected to Salmonella spp. from diarrheal disease patients and non-pathogenic Escherichia coli. Phage stability on thermal, pH, and buffer was then analyzed to determine the biological characteristics.    

Results: Three lytic phages (F-SB1, F-SB2, and F-SB3), successfully isolated from domestic wastewater, showed an icosahedral head with a short or long tail as their morphological characteristic. These phages were morphologically similar to the phages of family Siphoviridae, Myoviridae and Podoviridae. The three isolated lytic phages were stable at 27 ^°C to 37 ^°C, pH 4-7 in sodium magnesium buffer and effectively decreased the population of Salmonella spp., however could not lyse E. coli.       

Interpretation: All the isolated lytic phages in this study can contribute as cocktail phages in decreasing the population Salmonella spp.

Key words: Antibiotic resistant, Domestic wastewater, Lytic phages, Salmonellosis, Salmonella spp.

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