and I. Rusmana1
Biology, Faculty of Mathematics and Natural Science,
Bogor Agricultural University, Bogor,
16680, West Java, Indonesia
2Faculty of Science
State Islamic University of Raden Fatah,
Palembang, 30151, Indonesia
*Corresponding Author Email :
Aim: This study was
carried out to isolate and study the effectiveness of lytic phage from
domestic wastewater to reduce the population of Salmonella spp. in
patients suffering from diarrhea and to characterize biological phages.
Methodology: The lytic phages
from several domestic wastewater were identified using a transmission
electron microscope to know morphological phages. After identifying the
molecular weight protein by sodium dodecyl sulphate-polyacrylamide gel
electrophoresis, to know the effectiveness, the lytic phages were infected to
Salmonella spp. from diarrheal disease patients and non-pathogenic Escherichia
coli. Phage stability on thermal, pH, and buffer was then analyzed to
determine the biological characteristics.
Results: Three lytic
phages (F-SB1, F-SB2, and F-SB3), successfully isolated from domestic
wastewater, showed an icosahedral head with a short or long tail as their
morphological characteristic. These phages were morphologically similar to the
phages of family Siphoviridae, Myoviridae and Podoviridae. The three isolated
lytic phages were stable at 27 °C to 37 °C, pH 4-7 in
sodium magnesium buffer and effectively decreased the population of
Salmonella spp., however could not lyse E. coli.
Interpretation: All the isolated
lytic phages in this study can contribute as cocktail phages in decreasing
the population Salmonella spp.
Key words: Antibiotic resistant, Domestic wastewater, Lytic phages,
Salmonellosis, Salmonella spp.