and M. Bahadur1*
Molecular Biology Laboratory, Department of
Zoology, University of North Bengal, Darjeeling-734
Research Unit, Department of Zoology, University of North
Bengal, Darjeeling-734 013, India
*Corresponding Author Email :
Aim: The present study
was undertaken to understand the phylogenetic relationships of NPVs
(Nucleopolyhedroviruses) isolated from two pest species, Hyposidra talaca
and Biston (=Buzura) suppressaria. The phylogenetic
analyses based on the polyhedrin gene were assessed.
Methodology: Occlusion bodies
(OBs) were isolated separately from NPV infected dead Hyposidra talaca
and Buzura suppressaria larvae, and DNA was isolated from OBs. The polyhedrin
gene was amplified and sequenced followed by sequence divergence and
phylogenetic analyses using MEGA5.
Results: The phylogenetic
analyses based on the polyhedrin gene revealed that the NPV isolated
from Hyposidra talaca (HytaNPV-ITK1) formed a single cluster with the
isolates of NPVs infecting Hyposidra specimens in India sharing 99%
nucleotide identity, whereas the NPV isolated from Buzura suppressaria
(BusuNPV-ITK1) showing 99% nucleotide homology with the NPV isolate of B.
suppressaria reported from China formed a different cluster. A nucleotide
identity of 85% was found between HytaNPV-ITK1 and BusuNPV-ITK1.
analyses, based on the polyhedrin sequence of 47 baculoviruses, revealed that
these two variants of NPVs (HytaNPV-ITK1 and BusuNPV-ITK1) infecting Hyposidra
talaca and Buzura suppressaria were comparatively closer to each
other than those infecting specimens of other lepidopteran genera.
Key words: Buzura suppressaria, Hyposidra talaca, Nucleopolyhedrovirus, Phylogeny, Tea pest