JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status
    JEB Award

Search the Journal web-site through Google:

        Abstract - Issue Jan 2019, 40 (1)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Effect of heat stress on enzymatic and non-enzymatic antioxidants in Brassica rapa

Paper received: 16.05.2018                       Revised received: 06.07.2018                    Re-revised received: 14.07.2018                   Accepted: 30.07.2018




Authors Info

R.A. Wilson1,2*, S. Gupta1,

M.K. Sangha1,2 and G. Kaur1 


1Department of Plant Breeding   and Genetics, Punjab Agricultural University,

Ludhiana-141 004, India


2Department of Biochemistry, Punjab Agricultural University, Ludhiana-141 004, India 




*Corresponding Author Email :





Aim: Heat stress due to increase in global temperature is posing a serious threat to the agricultural sector in many parts of the world. The present investigation was, therefore, undertaken to study the mechanism of thermos-tolerance in four-day-old seedlings of Brassica rapa (44 genotypes) on the basis of various enzymatic and non-enzymatic antioxidants. The information gathered through the present investigation can pave way for imparting tolerance to Brassica genotypes by altering enzyme activities through genetic engineering interventions.  


Methodology: A total of 44 genotypes were evaluated for survival percentage, electrolyte leakage and chlorophyll content under heat stress conditions. Seedlings were characterized by membrane lipid peroxidation and antioxidants viz. peroxidase and catalase activities, proline and glutathione. Heat stress conditions were created by exposing four-day-old seedlings to 45șC for 4.5 hr. Out of 44 genotypes, four genotypes (JMT-04-03, TL-2035, TL-98-01 and PBT-37) were thermos-tolerant. Tolerant genotypes registered survival greater than 65%, moderately tolerant between 35-65% and susceptible less than 35%.        


Results: Among various parameters studied, under heat stress, a significant increase in electrolyte leakage, lipid peroxidation, peroxidase activity, glutathione and proline content was observed in comparison to control seedlings, whereas a decline in CAT activity and chlorophyll content was recorded.         


Interpretation: Biochemical changes observed in the activities and contents of various parameters studied could be linked with enhanced tolerance to heat stress damage in Brassica rapa which could further be used as a marker for screening against heat stress.


Key words: Antioxidants, Brassica rapa, Heat stress, Lipid Peroxidation, Reactive oxygen species



Copyright © 2019 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).