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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2018, 39 (5)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Growth characteristics of shade-acclimated marine Chlorella vulgaris under high-cell-density conditions


T. Katayama1, N. Nagao2*, M. Goto3, F. Md. Yusoff2, S. Banerjee2, M. Sato1, K. Takahashi1 and K. Furuya1

1Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan

2Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

3Faculty of Science and Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan

*Corresponding Author E-mail:




Key words

Chlorella vulgaris

high cell density

light intensity per cell

light-saturation index

shade acclimation




Publication Data

Paper received : 09.06.2017

Revised received : 25.10.2017

Re-revised received : 30.11.2017

Accepted : 28.12.2017



Aim: To potentially improve the productivity of microalgae for mass culture, growth characteristics and light-saturation index (Ek) in the marine microalga Chlorella vulgaris were investigated under high-cell-density conditions grown in batch cultures.


Methodology: Potential changes in the growth characteristics and Ek in C. vulgaris were investigated during 4 weeks of cultivation. The algae was cultivated in a column reactor at 25С under a surface light intensity of 300 μE m−2 s−1 in a 12-hr light and 12-hr dark cycle.


Results: Cell density increased and reached 2.7 g-dw l−1 by the end of culture with an areal production rate of 3.3 g-dw m−2 day−1. The specific growth rate reached a maximum of 0.41 day−1 when the light intensity per cell was 7.9 μE g-dw−1 s−1. As the light intensity per cell decreased with increasing cell density, Ek decreased gradually during the first half of the culture period.


Interpretation: Chlorella vulgaris in this study had the ability to acclimate to shade conditions during high-cell-density cultivation, however, the photosynthetic efficiency decreased. High transformation efficiency of light energy for photosynthesis in shade-acclimated cells will help to exploit the mass culture of microalgae.



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