characteristics of shade-acclimated marine Chlorella vulgaris under
Katayama1, N. Nagao2*, M. Goto3, F. Md.
Yusoff2, S. Banerjee2, M. Sato1, K.
Takahashi1 and K. Furuya1
1Graduate School of
Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo
Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia,
43400 Serdang, Selangor Darul Ehsan, Malaysia
3Faculty of Science
and Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan
Author E-mail: firstname.lastname@example.org
high cell density
light intensity per cell
Paper received : 09.06.2017
Revised received : 25.10.2017
Re-revised received :
Accepted : 28.12.2017
Aim: To potentially
improve the productivity of microalgae for mass culture, growth
characteristics and light-saturation index (Ek) in the marine
microalga Chlorella vulgaris were investigated under high-cell-density
conditions grown in batch cultures.
Methodology: Potential changes
in the growth characteristics and Ek in C. vulgaris were
investigated during 4 weeks of cultivation. The algae was cultivated in a
column reactor at 25°С under a surface light intensity of
300 μE m−2 s−1 in a 12-hr light and
12-hr dark cycle.
Results: Cell density
increased and reached 2.7 g-dw l−1 by the end of culture
with an areal production rate of 3.3 g-dw m−2 day−1.
The specific growth rate reached a maximum of 0.41 day−1
when the light intensity per cell was 7.9 μE g-dw−1 s−1.
As the light intensity per cell decreased with increasing cell density, Ek
decreased gradually during the first half of the culture period.
vulgaris in this study had the ability to acclimate to shade conditions
during high-cell-density cultivation, however, the photosynthetic efficiency
decreased. High transformation efficiency of light energy for photosynthesis
in shade-acclimated cells will help to exploit the mass culture of
© 2018 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s).