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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2018, 39 (5)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

The potential of bioflocculant-producing bacteria as

inoculum for biofloc based systems


A.A.C. Harun1, N.A. Ghazali1, N.F.C. Hashim1, N.A.H. Mohammad1, Mhd. Ikhwanuddin1,2, N. Ismail3, Z. Ibrahim4 and N.A. Kasan1,2*

1Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia

2School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia

3Institute of Marine Biotechnology, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia

4Department of Biological Science, Faculty of Bioscience and Bioengineering, Universiti Teknologi Malaysia, 81300, Skudai, Johor, Malaysia

*Corresponding Author E-mail:




Key words

bioflocculant-producing bacteria

extracellular polymeric substance

Flocculation activity

Priological flocculants




Publication Data

Paper received : 25.07.2017

Revised received : 20.08.2017

Re-revised received : 25.09.207

Accepted : 28.12.2017



Aim: Biological flocculants has been widely used around the world to replace the usage of synthetic flocculants for wastewater treatment. A new green technology using biological flocculant known as biofloc system was developed which offers zero water exchanged, low feed conversion ratio (FCR) and high nutritional profile. This study was conducted to determine the most potential bioflocculant-producing bacteria isolated from biofloc sample in Pacific Whiteleg shrimp, P. vannamei culture pond.     


Methodology: Biofloc sample was collected using Imhoff cone and bacteria was isolated. The most abundant bacteria isolated throughout P. vannamei culture period was selected for screening of bioflocculant-producing bacteria using YPG medium and flocculation activity using floc-jar test.      


Results: Screening of bioflocculant-producing bacteria showed that B. infantis, N. aquimarinus, B. cereus, H. venusta, Pseudoalteromonas sp., and B. safensis were characterized as highly mucoid and ropy colony morpologies. The highest flocculation activity with 93% was showed by B. infantis followed by N. aquimarinus (91%), B. cereus (87%), H. venusta (79%), Pseudoalteromonas (78%), while the lowest flocculation activity was showed by B. safensis with 69%.


Interpretation: As bacteria grew, extracellular polymeric substances (EPS) produced were involved in flocculation process. Each bacteria produced different EPS composition which differed their ability in flocculation process. Therefore, bacteria with high flocculation activity are potentially used as inoculum to increase flocculation process in biofloc production.




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