and population structure of Tenualosa toli inferred from Cytochrome b
mitochondrial DNA fragment
Puvanasundram1*, Y. B. Esa1, K.A.A. Rahim2
and S.M. Nurul Amin1
Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor Darul Ehsan, Malaysia
Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota
Samarahan, Sarawak, Malaysia
Author E-mail: email@example.com
Cyt b sequences
Paper received : 16.07.2017
Revised received : 19.09.2017
Re-revised received :
Accepted : 28.12.2017
Aim: Shad fish of genus
Tenualosa subfamily Alosinae (shads) family Clupeidae, are
commercially and culturally important estuarine fish in many Asian countries,
especially in Sarawak. In this study, the phylogenetics of three species from
the genus Tenualosa (Tenualosa toli, Tenualosa macrura and Tenualosa
ilisha) was determined.
Methodology: Sequence analysis
of 910 base pairs of Cytochrome b gene were conducted on the samples of
indigenous T.toli (N=111) and T. macrura (N=24), which were
collected from Sarawak including the samples of non-native T. ilisha
(N=4) obtained from Bangladesh.
Results: A total of 28
haplotypes were found with T. toli producing 15 haplotypes, where 13
haplotypes were unique haplotypes while 2 shared haplotypes among the 6
analysis supported the monophyletic status between the three shad species.
The highest intraspecific genetic divergences were recorded between imported
samples and samples from other localities. There is evidence of overfishing
and recently occurred bottleneck events which led to a population size
expansion of T. toli, especially in Sebuyau, Daro and Mukah.
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