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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2018, 39 (5)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Abrogation of sodium arsenite driven uterine antioxidant exhaustion and tissue impairment: Role of B12 and folate

 

B. Deb1, M. Maity1, S. Maiti2, B. Pan1, H. Perveen1, M. Dash1, A.K. Maiti3 and S. Chattopadhyay1*

1Department of Biomedical Laboratory Science and Management and Clinical Nutrition and Dietetics Division, Vidyasagar University, Midnapore-721 102, India

2Department of Biochemistry, Oriental Institute of Science and Technology, Midnapore-721 102, India

3Department of Pathology, Midnapore Medical College and Hospital, Midnapore-721 102, India

*Corresponding Author E-mail: sandipdoc@mail.vidyasagar.ac.in

 

 

 

Key words

Folic acid

S-adenosine methionine pool

sodium arsenite

uterine oxidative stress

vitamin B12

 

 

 

Publication Data

Paper received : 16.06.2017

Revised received : 23.10.2017

Re-revised received : 28.11.2017

Accepted : 28.12.2017

 

Abstract

Aim: Rapid emergence of arsenic pollution demands the development of novel adjunct with non-invasive painless oral therapeutic agent to combat against arsenic associated health hazards by replacing conventional painful chelating therapy. In the present study, the modulatory effects of vitamin B12 and folic acid (folate) in the amelioration of arsenic mediated uterine disorders were examined.

 

Methodology: In this experimental study, Wistar strain adult female rats were fed sodium arsenite (As3+) contaminated water (0.4 ppm) in conjunction with vitamin B12 (0.04 μg and 0.07 μg) plus folate (2.0 and 4.0 μg) alone and or in combination respectively per 100 g b.wt. per day for seven estrous cycles (28 days).

 

Results: Rats those underwent arsenic exposure showed significant impairments in the status of uterine antioxidant profile as evident from enzymatic assay and electrozymogram study of superoxide dismutase, catalase and peroxidase with an abnormal increase in conjugated diene and malondialdehyde levels. Mutagenic uterine DNA-breakage and tissue damages were prominent following As3+consumption by the rats. All impairments were totally or partially attenuated by the co-treatment with these two B vitamins in arsenic exposed rats.

 

Interpretation: The mechanisms might be coupled with the enhancement of antioxidant defense system, partly through the elimination of arsenic with the involvement of S-adenosine methionine pool (SAM) since, vitamin B12 and folic acid are two major regulators of this pool.

 

 

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