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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2018, 39 (5)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Identification and molecular characterization of root nodule microsymbiont of Trigonella foenum-graecum L. growing in different soils from Western Rajasthan, India


S. Gaur1, N. Tak1, S. Rathi1, S. Choudhary1,2 and H.S. Gehlot1*

1BNF and Microbial Genomics Lab., Department of Botany, UGC-Center of Advanced Study, Jai Narain Vyas University, Jodhpur - 342 001, India

2Forest Protection Division, Arid Forest Research Institute, Jodhpur - 342 005, India

*Corresponding Author E-mail:




Key words




Sym genes

Trigonella foenum-graecum



Publication Data

Paper received : 02.08.2017

Revised received : 06.12.2017

Re-revised received : 13.01.2018

Accepted : 06.02.2018



Aim: To identify and characterize N fixing root nodule bacteria (RNB) of Trigonella foenum-graecum (TFG) growing in soils collected from different districts of Western Rajasthan.


Methodology: Selected RNB strains were characterised phenotypically and molecular phylogeny was studied using their 16S rRNA and symbiosis-related gene sequences.


Results: The 31 selected RNB strains formed three groups on the basis of colony characteristics. The TFG-RNB strains showed metabolic diversity in terms of carbon utilization and intrinsic antibiotic resistance, with few strains showing NaCl tolerance up to 4% and growth on a wide range of pH (4-11). In 16S rRNA gene phylogeny six (TFG22, TFG33, TFG53, TFG59, TFG64 and TFG66) selected strains clustered in a novel clade close to type strains Ensifer medicae and Ensifer meliloti forming two distinct 16S rRNA types. The symbiotic (nodA and nifH) and 16S rRNA phylogenies of TFG-Ensifer strains were in congruence. The sym genes of TFG strains have intermediate sequences diversified from closely related E. meliloti and E. medicae which indicates their evolution through horizontal gene transfer.


Interpretation: Nitrogen fixing TFG-Ensifer strains isolated from Western Rajasthan are potentially novel and showing plant growth promoting activity therefore can be used as inoculants in agricultural fields of semi-arid regions.



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