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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Sep 2018, 39 (5)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

A study on induced breeding, embryonic and larval development of Pangasianodon hypophthalmus in semi-arid agro-climate


S.N. Datta*, A. Singh, G. Jassal and A. Pandey

College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana–141 004, India

*Corresponding Author E-mail:




Key words

Embryonic development

Induced breeding

Larval devlopment

Pangasianodon hypophthalmus Pituitary extract 



Publication Data

Paper received : 13.10.2017       

Revised received : 10.12.2017    

Re-revised received : 20.02.2018

Accepted : 04.04.2018



Aim: Breeding performance of Pangasianodon hypophthalmus in semi-arid agro-climatic conditions of northern India was evaluated in view of establishing hatchery to supply its seed. 


Methodology: P. hypophthalmus were induced to breed using carp pituitary gland (PG) extract. Females were administered with priming dose of 2.5-3.0 mg kg-1; after 6 hrs, a second resolving dose of 10-12 mg kg-1 was given, while males were administered with single dose of 2.5-3.0 mg kg-1 body weight. Eggs were dry fertilized after 14 hrs of second injection. The fertilized eggs were allowed to incubate in a circular hatchery maintaining a water flow of 30 l min-1. Embryonic and larval developments were assessed by observing eggs at regular interval under a microscope. Optimum water quality parameters were maintained during entire experiment period.


Results: The average fecundity was 57.5 lakh kg-1 of body while fertilization and hatching percentage was 76.50 and 58.20, respectively. After fertilization, eggs became water hardened within 20 min. “Blastodisc stage” appeared after 00:25 hr and “Morula stage” after 03:45 hr of fertilization. “Blastula stage” was observed after 05:30 hr post fertilization. Twitching movement started 24:00 - 26:00 hr post fertilization and hatching started at 34:00-36:00 hr post fertilization. A newly hatched larva measured about 3.80 mm in length with head slightly bent and compact yellowish yolk sac. During the age of 5-10 days of life, pectoral, caudal, ventral and dorsal fins appeared.  


Interpretation: Stripped catfish Pangasianodon hypophthalmus can be successfully and commercially bred in semi-arid agro-climatic conditions of northern India under controlled environmental conditions using PG as inducing agent. However, females were not observed to ovulate naturally but dry fertilization technique.



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