analyses of phytochemical composition and antimicrobial properties of
different solvent extracts of
Keskin1* and N.C. Guvensen2
High School, Adnan Menderes University, Aydın-09010, Turkey
Department, Faculty of Sciences, Muğla University, Muğla-48000,
Author E-mail: firstname.lastname@example.org
Paper received :
Revised received :
Re-revised received :
Accepted : 29.03.2018
Aim: The ethanol,
methanol, hexane, chloroform, isopropanol and water extract of Melissa
officinalis leaves were tested for antimicrobial activity against eleven
bacteria and one yeast by disc diffusion method.
Methodology: The antimicrobial
activity was assessed and the volatile components of chloroform and dimethyl
sulfoxide extracts of Melissa officinalis leaves were analysed by
Results: In the present
study, chloroform extracts of Melissa officinalis leaves showed the best
inhibition zone (27 mm) against Candida albicans. Inhibition zones of
the all the extracts varied from 8 to 27 mm against tested microorganisms.
The minimum inhibitory concentration (MIC) of M. officinalis subsp. officinalis
extracts obtained by broth serial dilution method, chloroform extracts were
among the most active with the MIC values ranging from 0.016-128 mg ml-1.
C. albicans showed lowest sensitivity to 0.016 mg ml-1
concentration of chloroform extracts. GC/MS analyses of dimethyl sulfoxide
extracts identified three compounds; the primary content of lemon balm leaves
extract was dimethyl sulfoxide (98.53%). Similarly, GC/MS analyses of
chloroform extract identified eight compounds; the primary contents of the of
lemon balm leaves extract were chloroform (96.6%).
Interpretation: Different solvent
extracts of Melissa officinalis leaves showed a broad-spectrum activity
against both Gram-positive and Gram-negative bacteria and fungi. Chloroform
and dimethyl sulfoxide extracts, especially showed better antimicrobial
activity due to high content of chloroform and dimethyl sulfoxide.
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