simple and efficient micropropagation protocol for New Guinea Impatiens (Impatiens
Samiei1*, M. Davoudi Panhehkolayi2, H. Mirshahi1
, and Z. Karimian1
Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University
of Mashhad, Mashhad, 91775-1653, Iran
Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad,
Author E-mail: email@example.com
Benzyl amino purine
Paper received : 10.07.2017
Revised received : 03.11.2017
Re-revised received :
Accepted : 22.12.2017
Aim: The study aimed to
compare the potentials for in vitro regeneration of apical and nodal
explants of New Guinea Impatiens in the presence of cytokinins and to develop
a simple, fast, cost-effective and efficient protocol for micropropagation of
this valuable plant species.
to high rate of contamination of Impatiens explants in in vitro
conditions, a preliminary experiment was performed on surface sterilization
of the bud explants using various sterilization reagents. In addition, the
effects of various concentrations of benzyl amino purine and kinetin on
proliferation of apical and axillary bud explants were investigated.
Following two subcultures in multiplication media, the plantlets with
well-developed roots were directly transferred to plastic cups and later to
the greenhouse for acclimatization.
Results: The most effective sterilization procedure, which
resulted in 75% healthy explants, was recognized when the bud explants were
disinfected with 0.1% mercuric chloride – 6 min – followed by a culture on
Murashig and Skoog medium, consisting of 300 mgl-1 Cefotaxime.
Also, the results revealed that axillary buds were more efficient in inducing
higher adventitious shoot regeneration compared to apical buds, when they
were exposed to 0.5 mg l-1 BAP in MS medium. In addition, the in
vitro roots were simultaneously induced during shoot proliferation and
maximum generated root number was observed in the medium containing 1 mg l-1
BAP). The results showed that about 95% of the regenerated plantlets
survived, acclimatized successfully and continued their normal and vigorous
growth in the greenhouse.
findings of this study indicated that the use of axillary buds in the
presence of low concentrations of BAP were effective in multiplication of New
Guinea Impatiens. The simultaneous occurrence of rooting during proliferation
was beneficial in significantly reducing the time and cost of
micropropagation procedure. The results propose a simple, fast, efficient and
economic protocol that can be easily commercialized for micropropagation of Impatiens
hawkeri 'Cherry Red'.
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