novel laccase producing Brevundimonas sp. MVSP from paper
pulp industry waste water
Poornima and Manickam Velan*
Chemical Engineering, A.C. College of Technology, Anna University,
Chennai-600 025, India
Author E-mail: firstname.lastname@example.org
Paper and pulp
Paper received : 17.06.2017
Revised received : 15.12.2017
Accepted : 30.12.2017
Aim: The present study
deals with the isolation, screening and characterization of laccase producing
bacterial strain from paper and pulp industry waste water. A novel laccase
producing bacterial strain Brevundimonas sp. MVSP was identified and
the optimum conditions required for maximal laccase enzyme activity was
dilution method was used for screening laccase producing strain. Out of 58
isolated microorganisms, seven positive strains were screened. Among the
seven isolates, “MVSP” showed highest enzyme activity towards laccase production.
The isolated microorganism was characterized by biochemical tests and
molecular analysis. The 16S rRNA sequence data were analysed and the results
were compared with Genbank database.
Results: Various operating parameters such as incubation time,
pH, temperature, carbon and nitrogen sources were optimized for
intensification of laccase activity. On the basis of laccase activity, Brevundimonas
genus was found to have higher laccase activity among the other isolates, and
was determined using phylogenetic analysis. The sequence received the Gen
Bank accession number KP712776. The crude enzyme isolated from Brevundimonas
sp. MVSP strain showed the maximum laccase activity of 5.24 U ml-1
at the optimum conditions.
is the first report which claims that Brevundimonas sp. MVSP produced
laccase enzyme using CuSO4 and guaiacol as an inducer. The novel Brevundimonas
sp. MVSP strain had achieved maximum laccase activity of 5.24 U ml-1
at optimized pH (6.5) and temperature (35°C) in 96 hr. This potent
organism could be a suitable candidate for the large-scale production of
laccase and it may be used to treat various industrial effluents from
textile, paper and pulp industry etc.
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