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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Jul 2018, 39 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Prevalence of community- acquired methicillin- resistant Staphylococcus aureus among tribal population of

north- western Himalayas, India

 

H. Kumar1*, U. Zahoor2, R. Rana2, T. Mahajan2, M. Premeshwari Devi2, R.K. Garg2, R. Kumar3, S. Khanna3,

H.N. Laskar3, P. Thakur3, K. Tongbram3, D. Dwivedi3 and R. Palaha4

1Faculty of Microbiology, Lovely Professional University, Phagwara -144 411, India

2Undergraduate Programme of Biotechnology, Lovely Professional University, Phagwara-144 411, India

3Undergraduate Programme of Microbiology, Lovely Professional University, Phagwara-144 411, India

4Department of Health, Civil Hospital, Bathinda-151 001, India

*Corresponding Author E-mail: harsh36yac@yahoo.com

 

 

 

Key words

CA-MRSA

Nasal colonization

Staphylococcus aureus

Tribal population

 

 

 

Publication Data

Paper received : 12.02.2017

Revised received : 08.08.2017

Re-revised received : 14.11.2017

Accepted : 24.11.2017??

 

Abstract

Aim: The objective of the study was to assess the percentage of community-acquired methicillin-resistant Staphylococcus aureus (CA -MRSA) and their antibiotic resistant patterns among the native population of north-western Himalaya regions, India. The study was conducted on three tribal communities namely Bakarwal, Gujjar and Gaddi of Jammu and Kashmir (J&K) and Himachal Pradesh (HP) states of India.?

 

Methodology: Identification and isolation of CA-MRSA was conducted by culturing it in the Mannitol Salt Agar and incubating at 37?C for 24 hrs. Single yellow colour colonies were selected and subjected to Gram staining as well other biochemical test such as, Coagulase, D-trehalose fermentation, maltose fermentation, DNase and β haemolysis test were performed. The positive CA-MRSA strains were then subjected to antibiotic susceptibility testing and statistical analysis was done.?

 

Results: In total, 1134 nasal swab samples were collected from healthy individuals for isolation of S. aureus and antibiogram was carried out for the screening of CA?MRSA; confirmed by oxacillin screen agar. Twenty four, CA-MRSA were isolated i.e., the frequency of nasal carriage CA-MRSA was 2.11% (24/1134). MRSA isolates were susceptible to gentamicin, doxycyclins hydrochloride, respectively.?     

 

Interpretation: CA-MRSA is highly prevalent in the young age groups of tribal population.

 

 

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