of community- acquired methicillin- resistant Staphylococcus aureus
among tribal population of
western Himalayas, India
Kumar1*, U. Zahoor2, R. Rana2, T. Mahajan2,
M. Premeshwari Devi2, R.K. Garg2, R. Kumar3,
Laskar3, P. Thakur3, K. Tongbram3, D.
Dwivedi3 and R. Palaha4
Microbiology, Lovely Professional University, Phagwara -144 411, India
Programme of Biotechnology, Lovely Professional University, Phagwara-144 411,
Programme of Microbiology, Lovely Professional University, Phagwara-144 411,
Health, Civil Hospital, Bathinda-151 001, India
Author E-mail: firstname.lastname@example.org
Paper received : 12.02.2017
Revised received : 08.08.2017
Re-revised received :
Accepted : 24.11.2017
Aim: The objective of
the study was to assess the percentage of community-acquired methicillin-resistant
Staphylococcus aureus (CA -MRSA) and their antibiotic resistant
patterns among the native population of north-western Himalaya regions,
India. The study was conducted on three tribal communities namely Bakarwal,
Gujjar and Gaddi of Jammu and Kashmir (J&K) and Himachal Pradesh (HP)
states of India.
and isolation of CA-MRSA was conducted by culturing it in the Mannitol Salt
Agar and incubating at 37°C for 24 hrs. Single yellow colour
colonies were selected and subjected to Gram staining as well other
biochemical test such as, Coagulase, D-trehalose fermentation, maltose
fermentation, DNase and β haemolysis test were performed. The positive
CA-MRSA strains were then subjected to antibiotic susceptibility testing and
statistical analysis was done.
total, 1134 nasal swab samples were collected from healthy individuals for
isolation of S. aureus and antibiogram was carried out for the screening of
CA–MRSA; confirmed by oxacillin screen agar. Twenty four, CA-MRSA were
isolated i.e., the frequency of nasal carriage CA-MRSA was 2.11% (24/1134).
MRSA isolates were susceptible to gentamicin, doxycyclins hydrochloride,
is highly prevalent in the young age groups of tribal population.