nanoparticles production by Aspergillus niger and their
antibacterial efficacy against Xanthomonas citri and
Devi Thokala*, D. Kamil and R.S. Toppo
Division of Plant
Pathology, Indian Agricultural Research Institute, New Delhi -110 012, India
Paper received : 18.10.2016
Revised received : 08.03.2017
Re-revised received :
Accepted : 02.11.2017
is being widely applied in multidisciplinary fields including biopesticide
applications. Recently, there is an increase research in the fungal mediated
production of silver and copper nanoparticles.
this investigation, the silver nanoparticles were synthesized from the
isolates of industrial fungus, Aspergillus niger and their efficacy
was examined using well diffusion assay against two phyto-pathogenic bacteria
viz., Xanthomonas citri and Ralstonia solanacearum. The silver
nanoparticles developed from Aspergillus niger cell filtrate were
described through UV-Vis spectrophotometer, Transmission electron microscope
(TEM) analysis and Fourier Transform Infrared Spectroscope (FTIR).
Results: The production of silver nanoparticles was confirmed
by visualization of peak at 420 nm in UV-Vis spectrophotometer. FTIR studies
showed the protein capping of these silver nanoparticles, while TEM
microphotographs showed well dispersed silver nanoparticles which are
isotropic and round having size between 3–25 nm. The 20-30 mm diameter
inhibition zones were formed by biosynthesized silver nanoparticles when tested
against Xanthomonas citri and Ralstonia solanacearum.
based silver nanoparticles were proved to be used against plant pathogenic
bacteria due to their broad spectrum efficacy. Further studies are needed to
investigate the efficacy of silver nanoparticles as antibacterial agent in
© 2018 Triveni Enterprises. All rights reserved. No part of the Journal can
be reproduced in any form without prior permission. Responsibility
regarding the authenticity of the data, and the acceptability of the
conclusions enforced or derived, rest completely with the author(s).