JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Home
    Obituary: Dr. R. C. Dalela
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
    About Triveni Enterprises
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
 

Google Search the Journal web-site:


    Abstract - Issue Jul 2018, 39 (4)                                     Back


nstantaneous and historical temperature effects on a-pinene

Evaluation of cellular induction, soluble components of proteins and expression of pro-inflammatory genes in Labeo rohita fingerlings

 

V. Kumar1,2*, K. Kumar2, R.P. Raman2, K.P. Prasad2, N. Kumar3, S. Kumar2, V. Kumar4 and S. Roy1,2

1Central Inland Fisheries Research Institute, Barrackpore-700 120, India

2Central Institute of Fisheries Education, Mumbai-400 061, India

3ICAR-National Institute of Abiotic Stress Management (NIASM), Baramati-413 115, India

4Division of Aquaculture, Kentucky State University, Frankfort, KY 40601, USA

*Corresponding Author E-mail: kumar.vika.vikash2@gmail.com

 

 

 

Key words

Acute inflammation

Carrageenan

Cellular markers

Inflammation mediators

 

 

Publication Data

Paper received : 06.06.2016

Revised received : 27.03.2017

Re-revised received : 27.09.2017

Accepted : 29.09.2017?????       

 

Abstract

Aim: To investigate the acute inflammatory response in Labeo rohita induced by carrageenan (red seaweeds) ?   

 

Methodology: The Labeo rohita fingerlings were injected with 50 μl of carrageenan and the inflammatory response was examined at 3, 6, 12, 24, 48 and 96 hr post injection. The cellular markers that includes thrombocyte, macrophage, monocyte, granulocyte (neutrophils) and lymphocyte counts, serum myeloperoxidase activity, nitroblue tetrazolium (NBT) assay, total protein, albumin and globulin, C-reactive protein (CRP) and cortisol were examined. Additionally, the mRNA expression of proinflammatory cytokine genes IL-1β and TNF-α and complement (C3) were analyzed by real time PCR.        

 

Results: Analysis of fish fingerlings showed significantly (p < 0.05) higher thrombocyte and macrophage count in the inflammatory exudates of carrageenan injected group. The neutrophil and monocyte number were correlated with the inflammatory response and higher counts were observed during 12 hrs and beyond, whereas lymphocyte counts were lower in the treated group as compared to control. The NBT assay and myeloperoxidase activity that indicates the neutrophils activation were significantly (p < 0.05) upregulated in the treatment group. The C- reactive protein (acute phase protein), globulin, proinflammatory genes, i.e., IL-1β, TNF-α and complement C3 were recorded significantly (p < 0.05) higher in the fishes injected with carrageenan as compared to control. ??          

 

Interpretation: The carrageenan induces acute inflammatory response in Labeo rohita and the results provide a basic experimental model to further study the activity of anti-inflammatory drugs and mediators of inflammation.

 

 

Copyright ? 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).