of cellular induction, soluble components of proteins and expression of
pro-inflammatory genes in Labeo rohita fingerlings
Kumar1,2*, K. Kumar2, R.P. Raman2, K.P.
Prasad2, N. Kumar3, S. Kumar2, V. Kumar4
and S. Roy1,2
Fisheries Research Institute, Barrackpore-700 120, India
of Fisheries Education, Mumbai-400 061, India
Institute of Abiotic Stress Management (NIASM), Baramati-413 115, India
Aquaculture, Kentucky State University, Frankfort, KY 40601, USA
Paper received : 06.06.2016
Revised received : 27.03.2017
Re-revised received :
Accepted : 29.09.2017
Aim: To investigate the
acute inflammatory response in Labeo rohita induced by carrageenan
Labeo rohita fingerlings were injected with 50 μl of carrageenan
and the inflammatory response was examined at 3, 6, 12, 24, 48 and 96 hr post
injection. The cellular markers that includes thrombocyte, macrophage,
monocyte, granulocyte (neutrophils) and lymphocyte counts, serum
myeloperoxidase activity, nitroblue tetrazolium (NBT) assay, total protein, albumin
and globulin, C-reactive protein (CRP) and cortisol were examined.
Additionally, the mRNA expression of proinflammatory cytokine genes
IL-1β and TNF-α and complement (C3) were analyzed by real time PCR.
Results: Analysis of fish fingerlings showed significantly (p
< 0.05) higher thrombocyte and macrophage count in the inflammatory
exudates of carrageenan injected group. The neutrophil and monocyte number
were correlated with the inflammatory response and higher counts were
observed during 12 hrs and beyond, whereas lymphocyte counts were lower in
the treated group as compared to control. The NBT assay and myeloperoxidase
activity that indicates the neutrophils activation were significantly (p
< 0.05) upregulated in the treatment group. The C- reactive protein (acute
phase protein), globulin, proinflammatory genes, i.e., IL-1β, TNF-α
and complement C3 were recorded significantly (p < 0.05) higher in
the fishes injected with carrageenan as compared to control.
carrageenan induces acute inflammatory response in Labeo rohita and
the results provide a basic experimental model to further study the activity
of anti-inflammatory drugs and mediators of inflammation.
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