Influence
of bud sett soaking with ethephon and its foliar application on growth,
biochemical attributes and
gene
expression in sugarcane
R.
Jain*, A. Singh, S.P. Singh and A. Chandra
Plant Physiology
and Biochemistry Division, ICAR- Indian Institute of Sugarcane Research, Lucknow-226
002, India
*Corresponding Author
E-mail: radha_dinesh@yahoo.co.in
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Key
words
Bud sprouting
Ethephon
Gene expression
Sett priming
Sugarcane
Publication Data
Paper received : 06.02.2017
Revised received : 05.07.2017
Re-revised received :
11.12.2017
Accepted : 13.12.2017?????
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Abstract
Aim: The present study
was carried out to evaluate the effect of sett soaking and foliar application
of Ethephon treatment on sprouting, early growth characteristics, SAI and SPS
gene expression in bud and leaf tissues and important physio-biochemical
parameters which essentially regulate the process of shoot and root growth in
sugarcane.
Methodology:
Single
bud setts of sugarcane were initially primed with graded level of Ethephon
viz., 0, 100, 200 and 400 ppm for 24 hrs along with untreated control and
planted in soil tray culture under net house. Data on bud sprouting, growth
attributes were determined after 10 days. About 45 days after planting,
second application of Ethephon was performed through foliar spray. Data on
plant growth, sugar content, photosynthetic pigments, proline, total phenol
and activity of peroxidase and nitrate reductase were estimated. SAI and SPS
gene expression was also studied using gene specific primer pairs through
qRT-PCR.
Results: Ethephon treatment showed early and higher rate of
bud sprouting, improved plant vigor, stalk height, fresh weight of root and
shoot. Plants exposed to Ethephon exhibited higher chlorophyll content,
proline, total phenol contents, activity of soluble acid invertase, nitrate
reductase in vivo and peroxidase enzymes as compared to untreated
plants. SAI and SPS gene expression was upregulated in treated bud and leaf
tissues, respectively. ?
Interpretation:
Ethephon
showed stimulatory effects on bud sprouting and early growth characteristics
by modulating sugar hydrolysis and its transport to growing shoot and
improving photosynthetic pigments and activity of nitrate assimilating enzyme
in sugarcane plants.
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