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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jul 2018, 39 (4)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Effect of nitrogen and phosphorus on growth and microcystin production in three Microcystis species

 

K.L. Lee1, K.Y. Jung2, J.H. Kim4, Y.S. Kim3 and H.S. Kim3*

1Watershed Ecology Research Team, National Institute of Environmental Research, Incheon, 22689, South Korea

2Nakdong River Environment Research Center, National Institute of Environmental Research, Gyeongsangbuk-do, 40103, South Korea

3Department of Biology, Kyungpook National University, Daegu, 41566, South Korea

4Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, South Korea

*Corresponding Author E-mail: kimhsu@knu.ac.kr

 

 

 

Key words

Cyanobacterial blooms

Freshwater ecosystem

Microcystin

Microcystis species

Nitrogen & Phosphorus

 

 

 

Publication Data

Paper received : 08.04.2017

Revised received : 13.07.2017

Re-revised received : 06.09.2017

Accepted : 29.09.2017

 

Abstract

Aim: The effects of nitrogen and phosphorus concentration on growth and microcystin production were investigated in three species of bloom-forming Microcystis isolated from two South Korean freshwater systems.

 

Methodology: Three species of cyanobacteria were collected from Yeongchun Dam and Ankei Dam in Kyungpook Province, South Korea. Culture experiments were conducted at 25 1C under cool white fluorescent light (ca. 50 μmol m-2 s-1) in media with different concentrations of nitrogen (0 to 20 mg l-1) and phosphorus (0 to 5 mg l-1). Cell numbers were determined in a hemocytometer for calculation of growth rate. Microcystin was analysed using high pressure liquid chromatography.

 

Results: The highest growth rate (μmax) and maximal microcystin production occurred at nitrogen concentrations of 10 and 20 mg l-1 in all three species. The response to phosphorus concentration was more complex. The highest growth rate (μmax) of M. aeruginosa, M. ichthyoblabe and M. viridis occurred at phosphorus concentrations of 0.5 mg l-1, 0.1 mg l-1, and 3 mg l-1, respectively. M.aeruginosa also had maximal microcystin production at 0.5 mg l-1 P. In contrast, M.ichthyoblabe and M. viridis had high microcystin production at 0 mg l-1 and at 5 mg l-1 P (the highest tested concentration), and low microcystin production at 0.1 mg l-1 P. Thus at 0.1 mg l-1, M.ichthyoblabe had the highest growth rate but produced least amount of microcystin. The types of microcystins produced varied according to species and nutrient conditions.    

 

Interpretation: Microcystin production and growth in Microcystis species isolated from South Korea varied according to species and nutrient conditions. These species responded similarly to different nitrogen concentrations, but differently to different phosphorus concentrations.

 

 

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