on potential application of crude keratinase enzyme from Stenotrophomonas
sp. for dehairing in leather processing industry
Swornakumari, S. Meignanalakshmi*, R. Legadevi and A. Palanisammi
Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and
Animal Sciences University, Chennai-600 007, India
Controlled drug release
Paper received : 06.09.2016
Revised received : 25.11.2016
Re-revised received :
Accepted : 10.10.2017
poly-3-hydroxybutyrate is one of the most common biopolymer which is used in
different fields such as medicine, agriculture, textile, industrial and food
packaging. PHB microspheres are useful for targeting drugs to specific
infection sites and for prolonged drug release. The present study focus on
microsphere preparation for effective controlled drug release using
poly-3-hydroxy butyrate biopolymer.
the present study, poly-3-hydroxy butyrate microspheres were prepared using
solvent evaporation technique and characterized by SEM and FTIR.
Poly-3-hydroxy butyrate microspheres were encapsulated with BSA and
gentamicin. Drug encapsulation efficiency was determined. In vitro
drug release profile and in vitro cytotoxicity was also studied.
prepared microspheres were of 2µm in size. Microspheres fabricated with 1%
polyvinylalcohol showed encapsulation efficiency of 94.3% and 90.27% with BSA
and gentamicin, respectively. The in vitro release studies in
simulated body fluid, phosphate buffered saline and contact lens solution
showed initial burst release followed by controlled release. In vitro
cytotoxicity analysis showed 98% and 95% viability of cells in 3T3L1 cell
line for microsphere encapsulated with BSA and gentamicin, respectively.
butyrate microspheres were found to release BSA and gentamicin in a
controlled manner and were found to be non-toxic by in vitro
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