response of Culex pipiens larvae to sublethal concentrations of sodium
and calcium hypochlorite
Shu1, M. Shahen1,2*, M.A.M. Hegazi2, I.M. Al-Sharkaw2
and A.I. Seif2
1College of Life
Science, Northwest A & F University, Yangling, Shaanxi, 712 100, China
Department, Faculty of Science, Tanta University Tanta, 31527, Egypt
Author E-mail: Mshahen@science.tanta.edu.eg
Paper received : 03.08.2017
Revised received :
Re-revised received :
Accepted : 28.11.2017
Aim: The present study
investigated the physiological responses and histopathological changes of Culex
pipiens larvae exposed to sublethal concentrations of sodium and calcium
concentration of total protein, lipids and carbohydrates as well as activity
of acetylcholinesterase were determined in larvae using colorimetric methods.
The interaction between hypochlorite and fourth instars larvae of C.
pipiens was followed through histological sections.
contents of total protein and total carbohydrates of all larval instars
increased following treatment with sublethal concentrations of both
hypochlorite compounds. However, treatment of larvae with 1/10 LC50
induced significant reduction in the total carbohydrate content. In addition,
treatment of larvae with sublethal concentrations of both hypochlorite
compounds induced significant reduction in the total lipid content.
Furthermore, the acetylcholinesterase activity significantly decreased in
treated first and second larval instars, while increased in treated third and
fourth larval instars.
larvae treated with sublethal concentrations of calcium and sodium
hypochlorite, the mid gut cells appeared thinner and flattened, with
disappearance of the peritrophic matrix and reduced fat body. The present
study suggested that hypochlorite compounds had a pronounced effect on larval
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