JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
 
Read Journal
    Current Issue
    Journal Archives
 
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status
 

Search the Journal web-site through Google:


        Abstract - Issue May 2018, 39 (3)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Physiological response of Culex pipiens larvae to sublethal concentrations of sodium and calcium hypochlorite

 

Z. Shu1, M. Shahen1,2*, M.A.M. Hegazi2, I.M. Al-Sharkaw2 and A.I. Seif2

1College of Life Science, Northwest A & F University, Yangling, Shaanxi, 712 100, China

2Zoology Department, Faculty of Science, Tanta University Tanta, 31527, Egypt

*Corresponding Author E-mail: Mshahen@science.tanta.edu.eg

 

 

 

Key words

Acetylcholinesterase

Culex pipiens

Histopathological changes

Physiological response

Sodium hypochlorite

 

 

 

Publication Data

Paper received : 03.08.2017

Revised received : 10.10.2017

Re-revised received : 24.11.2017

Accepted : 28.11.2017

 

Abstract

Aim: The present study investigated the physiological responses and histopathological changes of Culex pipiens larvae exposed to sublethal concentrations of sodium and calcium hypochlorite.

 

Methodology: The concentration of total protein, lipids and carbohydrates as well as activity of acetylcholinesterase were determined in larvae using colorimetric methods. The interaction between hypochlorite and fourth instars larvae of C. pipiens was followed through histological sections.

 

Results: The contents of total protein and total carbohydrates of all larval instars increased following treatment with sublethal concentrations of both hypochlorite compounds. However, treatment of larvae with 1/10 LC50 induced significant reduction in the total carbohydrate content. In addition, treatment of larvae with sublethal concentrations of both hypochlorite compounds induced significant reduction in the total lipid content. Furthermore, the acetylcholinesterase activity significantly decreased in treated first and second larval instars, while increased in treated third and fourth larval instars.  

 

Interpretation: In larvae treated with sublethal concentrations of calcium and sodium hypochlorite, the mid gut cells appeared thinner and flattened, with disappearance of the peritrophic matrix and reduced fat body. The present study suggested that hypochlorite compounds had a pronounced effect on larval metabolic pathways.

 

 

Copyright 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).