JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editorial Board
    Reviewer Panel
    RnD Division
    Subscription Info
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Authoring Guidelines
    Publication Process
    Track Paper Status
    JEB Award

Search the Journal web-site through Google:

        Abstract - Issue May 2018, 39 (3)                                                                                                             Back

nstantaneous and historical temperature effects on a-pinene

Interspecies diversity of Bacillus thuringiensis isolates native from North Western Indian Himalayas


A.R.N.S. Subbanna1,2*, M.S. Khan1, R.M. Srivastava1, P.K. Mishra2, B. Kalyana Babu1 and V. Venkateswarlu3

1Department of Entomology, College of Agriculture, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar- 263 145, India

2ICAR- Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora -263 601, India

3Department of crop protection, ICAR-Central Tobacco Research Institute, Rajahmundry-533 105, India

*Corresponding Author E-mail:




Key words


Bacillus thuringiensis (Bt)

Genetic diversity

Gyrase B-sequence





Publication Data

Paper received : 30.01.2017

Revised received : 22.05.2017

Re-revised received : 16.09.2017

Accepted : 26.09.2017



Aim: Bacillus thuringiensis (Bt) is a ubiquitous pathogenic bacterium exploited worldwide in successful insect pest management programs. The present study aimed at resolving the diversity status and genetic structure of Bt isolates collected from unique and native ecological niche, north-western Indian Himalayas.


Methodology: Forty five Bacillus thuringiensis (Bt) isolates native to Uttarakhand, India were analyzed for their genetic diversity using Arbitrary Primed-Polymerase Chain Reaction (AP-PCR) patterns (ERIC1, ERIC2, BOX and GTG) and gyrase B (gyrB) sequences.


Results: Amongst the AP-PCR patterns, ERIC and BOX primers clearly indicated the genetic inconsistency of Bt by differentiating low and high hill isolates with unique banding pattern. A dendrogram generated showed fastidious clustering with coexistence of attitudinally related strains, indicating the genetic relatedness with respect to geographical proximity. The gyrB (285 bp) dependent phylogeny of seven selected isolates revealed their genetic proximity with different serovars.  


Interpretation: The present study demonstrates that ERIC and BOX-PCR can be used in establishment of geographical variation and gryB in phylogenetic relatedness with serovars. Genetic relatedness between Bt isolates was found to be a function of geographical proximity.



Copyright 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).