Interspecies
diversity of Bacillus thuringiensis isolates native from North Western
Indian Himalayas
A.R.N.S.
Subbanna1,2*, M.S. Khan1, R.M. Srivastava1,
P.K. Mishra2, B. Kalyana Babu1 and V. Venkateswarlu3
1Department of
Entomology, College of Agriculture, Govind Ballabh Pant University of Agriculture
and Technology, Pantnagar- 263 145, India
2ICAR- Vivekananda
Parvatiya Krishi Anusandhan Sansthan, Almora -263 601, India
3Department of crop
protection, ICAR-Central Tobacco Research Institute, Rajahmundry-533 105,
India
*Corresponding
Author E-mail: subbanna.ento@gmail.com
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Key
words
AP-PCR
Bacillus thuringiensis (Bt)
Genetic diversity
Gyrase B-sequence
Serovars
Publication Data
Paper received : 30.01.2017
Revised received : 22.05.2017
Re-revised received :
16.09.2017
Accepted : 26.09.2017
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Abstract
Aim: Bacillus
thuringiensis
(Bt) is a ubiquitous pathogenic bacterium exploited worldwide in successful
insect pest management programs. The present study aimed at resolving the
diversity status and genetic structure of Bt isolates collected from unique
and native ecological niche, north-western Indian Himalayas.
Methodology:
Forty
five Bacillus thuringiensis (Bt) isolates native to Uttarakhand, India
were analyzed for their genetic diversity using Arbitrary Primed-Polymerase
Chain Reaction (AP-PCR) patterns (ERIC1, ERIC2, BOX and GTG) and gyrase B
(gyrB) sequences.
Results:
Amongst
the AP-PCR patterns, ERIC and BOX primers clearly indicated the genetic
inconsistency of Bt by differentiating low and high hill isolates with
unique banding pattern. A dendrogram generated showed fastidious clustering
with coexistence of attitudinally related strains, indicating the genetic
relatedness with respect to geographical proximity. The gyrB (285 bp)
dependent phylogeny of seven selected isolates revealed their genetic
proximity with different serovars. ???
Interpretation:
The
present study demonstrates that ERIC and BOX-PCR can be used in establishment
of geographical variation and gryB in phylogenetic relatedness with
serovars. Genetic relatedness between Bt isolates was found to be a
function of geographical proximity.
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conclusions enforced or derived, rest completely with the author(s).
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