of virus free planting material through meristem culture in short day garlic
cultivars Bhima Omkar and Bhima Purple
Murkute1, 2* and S.J. Gawande1
1ICAR - Directorate
of Onion and Garlic Research, Rajgurunagar - 410 505, India
2ICAR - Central
Citrus Research Institute, Nagpur - 440 033, India
Author E-mail: firstname.lastname@example.org
Meristem tip culture
Paper received : 29.12.2016
Revised received : 12.05.2017
Re-revised received :
Accepted : 16.10.2017
Aim: Low productivity
of garlic in India has been attributed to viral infestation of planting
material. In vitro techniques offer a suitable alternative; hence,
standardization of protocol to raise virus free planting material through
meristem culture in vitro and its multiplication was planned.
of different cytokinins viz. BAP, Kin (1 and 1.5 mgl-1) in
combination with NAA (0.1, 0.5 mgl-1) on basal MS medium was
evaluated for inducing multiple shooting through meristem tip (0.1 – 0.3 mm)
in vitro in two garlic cultivars viz. Bhima Purple and Bhima Omkar. Higher
sucrose (6 to 11 %) along with cytokinins was evaluated for in vitro
microbulbils induction. Viral load was tested at different stages using
potyvirus specific alkaline phosphatase based direct antigen coating ELISA
kit. In vitro raised microbulbils were transferred to three production
cycles to get normal size garlic bulb.
+ 1 mgl-1 Kin + 0.1 mgl-1 NAA medium was the best
performing medium for induction of multiple shooting in both the cultivars.
About 70% mericlones were free from viruses in both the cultivars. Liquid
medium MS + 1 mgl-1 Kin + 6% sucrose produced the highest number
of microbulbils than other treatments. Normal garlic bulbs (9-12 cloves/bulb)
were produced in field from in vitro raised microbulbils after two
cycles of production in polyhouse followed by field transplanting.
meristem culture is an efficient method for producing virus free planting
material of garlic. Protocol for production of higher number of microbulbils in
vitro has also been standardized. Using standardized protocol a normal
size garlic bulb can be produced in three production cycles.
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