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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue May 2018, 39 (3)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Novel male sterile line development through marker-assisted backcross breeding suitable for aerobic planting in rice

 

P. Raghavendra* and S. Hittalmani

Marker Assisted Selection Laboratory, Department of Genetics and Plant Breeding, University of Agricultural Sciences, GKVK, Bangalore-560 065, India

*Corresponding Author E-mail: raghavendra9078@gmail.com

 

 

 

Key words

Aerobic condition

Male sterile line

Marker-assisted backcross

Rice hybrid

SSR markers

 

 

 

Publication Data

Paper received : 30.11.2016

Revised received : 16.02.2017

Re-revised received : 27.09.2017

Accepted : 29.09.2017

 

Abstract

Aim: Availability of suitable hybrids for drought or cultivation under aerobic situation is scarce in rice. Therefore, there is a dire need for developing of potential male sterile line for the production of promising hybrids. In this way, present study conducted was development of male sterile line suitable for aerobic rice hybrids in limited generations with the use of marker assisted backcrossing.

 

Methodology: The present study was conducted using strategy of marker assisted background selection for the development of new malesterile line in rice. A male sterile line KCMS31A was used as a donor parent for male sterility and a promising genotype MAS99 (early maturing and suitable for aerobic condition) as a recurrent parent. Backcrossing was carried out from 2013 to 2014. Phenotypic foreground selection was carried out in each generation by pollen fertility test. Based on parental polymorphism survey, 96 polymorphic SSR markers were identified and utilised for the marker assisted background selection in BC3F1 generation. Further, BC4F1 plants were evaluated for morphological characters.

 

Results: Analysis of data showed that all the plants were totally male sterile and the plants coded as BC3F1-5-7 (96.88%), BC3F1-5-10 (96.88%), BC3F1-5-15 (96.88%) and BC3F1-5-17 (95.83%) were found to be recovered maximum recurrent parent genome and they were advanced to BC4F1. Evaluation of new BC4F1 male sterile line pertaining to morphological traits revealed that the trait means were on par with the original fertile counterpart MAS 99 B line.  

 

Interpretation: The results confirmed the supremacy of marker assisted selection in backcross breeding aimed at the recovery of recurrent parent genome as quickly as possible and this is the first effort made by authors to develop male sterile line fit to utilise in hybrid rice programme under aerobic condition.

 

 

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