of amylase for wastewater treatment from Penicillium sp. SP2 isolated
from stagnant water
S. Priya1* and A. Renu2
Biochemistry, Sathyabama University, Chennai- 600 119, India
Biotechnology, Udaya School of Engineering, Kanyakumari-629 204, India
Author E-mail: firstname.lastname@example.org
Jack fruit seed
Solid substrate fermentation
Paper received : 18.10.2016
Revised received : 28.01.2017
Re-revised received :
Accepted : 27.06.2017
Aim: Amylolytic enzymes
are useful in bakery, food, automation dishwashing, fodder, wastewater
treatment and poultry. The main objective of the present study was to utilize
jack fruit seed as a cheap substrate for the production of amylase in solid
substrate fermentation using Penicillium sp. SP2 and to treat
water was used to isolate Penicillium sp. SP2 for amylase production.
The potent fungal isolate, Penicillium sp. SP2 was cultured in solid
substrate fermentation using jack fruit seed as a low cost substrate. The
process parameters were optimized to increase the production of enzyme.
Amylase was initially fractionated using ammonium sulphate and purified using
sephadex G-75 gel filtration column. The crude amylase from Penicillium
sp. SP2 was applied to treat wastewater.
isolated Penicillium sp. SP2 utilized jack fruit seed substrate and
produced amylase. Among the carbon sources, glucose significantly increased
the production of enzyme. Beef extract increased amylase production than
other nitrogen source. Ca2+ ion also significantly enhanced the
production of amylase. Amylase production was found to be maximum at 35 °C.
The molecular weight of purified amylase was 57 kDa as judged by SDS - PAGE.
The crude enzyme reduced carbohydrate content in wastewater and increased
dissolved oxygen considerably.
cheap cost, availability, and nutritive value, jack fruit seed is a novel
substrate for amylase production for various biotechnological applications.
The amylase from Penicillium sp. SP2 find great application in
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