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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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    Abstract - Issue Mar 2018, 39 (2)                                     Back


nstantaneous and historical temperature effects on a-pinene

Immunomodulatory activity of commercial β-glucan in

murine macrophage cell line RAW 264.7

 

E.Y. Choi1, J.Y. Jin1, J.Y. Hyeon1, S.H. Choe1, B.R. Keum1, J.M. Lim2, D.C. Park2, K.K. Cho3 and I.S. Choi1*

1Department of Life Science, Silla University, Busan, 46958, Republic of Korea

2Glucan Corporation, Busan Technopark, Marine Bioindustry Development Center, Busan, 46048, Republic of Korea

3Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju, 52725, Republic of Korea

*Corresponding Author E-mail: ischoi@silla.ac.kr

 

 

 

Key words

β-glucan

Cytokine

Immunomodulation

Macrophages

Tumor necrosis factor

 

 

 

Publication Data

Paper received : 01.09.2016

Revised received : 23.03.2017?????????? Re-revised received : 08.08.2017

Accepted : 07.09.2017          

 

Abstract

Aim: β-glucan, a cell wall component of a variety of fungi, yeasts and bacteria, has a regulatory potential for various diseases such as infection and inflammation. The present study investigated the effects of β-glucan (polycan) on immune modulation in murine macrophage RAW264.7 cells.

 

Methodology: As immune response parameters, production of nitric oxide (NO), reactive oxygen species (ROS) and cytokines like tumor necrosis factor (TNF)-a, interleukin (IL)-1β and IL-6 were assessed. iNOS protein expression, phosphorylation of mitogen-activated protein kinases (MAPKs), degradation of inhibitory κB-a (IκB-a), nuclear translocation of nuclear factor-κB (NF-κB) subunits and phosphorylation of signal transducer and activator of transcription 1 (STAT1) and STAT3 were characterized via immunoblotting.

 

Results: Production of NO, TNF-a, IL-1β and IL-6 were meaningfully increased in β-glucan (polycan) treated RAW264.7 cells, along with the increased expression of inducible NO synthase, TNF-a, IL-6 and IL-1β mRNA at concentrations with no cytotoxicity. β-glucan (polycan) treatment also caused the NF-κB and phosphorylation of MAPKs, STAT1 and STAT3, showing β-glucan (polycan) activated macrophages through activation of NF-κB and MAPKs signaling pathways in RAW264.7 cells.    

 

Interpretation: These results reveal the therapeutic effects of β-glucan (polycan) may partly be due to its ability to modulate immune functions in macrophages.

 

 

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