JEB logo

Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

About Journal
    Editor in Chief
    Editorial Board
    Reviewer Panel
    Publication Policies
    Guidelines for Editors
    Guidelines for Reviewers
    Abstracting and Indexing
    Subscription and Payments
    Contact Journal
Read Journal
    Current Issue
    Journal Archives
For Authors
    Guidelines for Authors
    Terms and Conditions
    Fees and Payments
    Track Paper Status
    JEB Awards

Google Search the Journal web-site:

    Abstract - Issue Mar 2018, 39 (2)                                     Back

nstantaneous and historical temperature effects on a-pinene

Immunomodulatory activity of commercial β-glucan in

murine macrophage cell line RAW 264.7


E.Y. Choi1, J.Y. Jin1, J.Y. Hyeon1, S.H. Choe1, B.R. Keum1, J.M. Lim2, D.C. Park2, K.K. Cho3 and I.S. Choi1*

1Department of Life Science, Silla University, Busan, 46958, Republic of Korea

2Glucan Corporation, Busan Technopark, Marine Bioindustry Development Center, Busan, 46048, Republic of Korea

3Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju, 52725, Republic of Korea

*Corresponding Author E-mail:




Key words





Tumor necrosis factor




Publication Data

Paper received : 01.09.2016

Revised received : 23.03.2017?????????? Re-revised received : 08.08.2017

Accepted : 07.09.2017          



Aim: β-glucan, a cell wall component of a variety of fungi, yeasts and bacteria, has a regulatory potential for various diseases such as infection and inflammation. The present study investigated the effects of β-glucan (polycan) on immune modulation in murine macrophage RAW264.7 cells.


Methodology: As immune response parameters, production of nitric oxide (NO), reactive oxygen species (ROS) and cytokines like tumor necrosis factor (TNF)-a, interleukin (IL)-1β and IL-6 were assessed. iNOS protein expression, phosphorylation of mitogen-activated protein kinases (MAPKs), degradation of inhibitory κB-a (IκB-a), nuclear translocation of nuclear factor-κB (NF-κB) subunits and phosphorylation of signal transducer and activator of transcription 1 (STAT1) and STAT3 were characterized via immunoblotting.


Results: Production of NO, TNF-a, IL-1β and IL-6 were meaningfully increased in β-glucan (polycan) treated RAW264.7 cells, along with the increased expression of inducible NO synthase, TNF-a, IL-6 and IL-1β mRNA at concentrations with no cytotoxicity. β-glucan (polycan) treatment also caused the NF-κB and phosphorylation of MAPKs, STAT1 and STAT3, showing β-glucan (polycan) activated macrophages through activation of NF-κB and MAPKs signaling pathways in RAW264.7 cells.    


Interpretation: These results reveal the therapeutic effects of β-glucan (polycan) may partly be due to its ability to modulate immune functions in macrophages.



Copyright ? 2018 Triveni Enterprises. All rights reserved. No part of the Journal can be reproduced in any form without prior permission. Responsibility regarding the authenticity of the data, and the acceptability of the conclusions enforced or derived, rest completely with the author(s).