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Abstract - Issue Mar 2018, 39 (2) Back
nstantaneous and historical temperature effects on a-pinene
activity of commercial β-glucan in
macrophage cell line RAW 264.7
Choi1, J.Y. Jin1, J.Y. Hyeon1, S.H. Choe1,
B.R. Keum1, J.M. Lim2, D.C. Park2, K.K. Cho3
and I.S. Choi1*
1Department of Life
Science, Silla University, Busan, 46958, Republic of Korea
Corporation, Busan Technopark, Marine Bioindustry Development Center, Busan,
46048, Republic of Korea
Animal Resources Technology, Gyeongnam National University of Science and
Technology, Jinju, 52725, Republic of Korea
Author E-mail: firstname.lastname@example.org
Tumor necrosis factor
Paper received : 01.09.2016
Revised received : 23.03.2017??????????
Re-revised received : 08.08.2017
Accepted : 07.09.2017
Aim: β-glucan, a
cell wall component of a variety of fungi, yeasts and bacteria, has a
regulatory potential for various diseases such as infection and inflammation.
The present study investigated the effects of β-glucan (polycan) on
immune modulation in murine macrophage RAW264.7 cells.
immune response parameters, production of nitric oxide (NO), reactive oxygen
species (ROS) and cytokines like tumor necrosis factor (TNF)-a, interleukin (IL)-1β and IL-6
were assessed. iNOS protein expression, phosphorylation of mitogen-activated
protein kinases (MAPKs), degradation of inhibitory κB-a (IκB-a), nuclear translocation of nuclear
factor-κB (NF-κB) subunits and phosphorylation of signal transducer
and activator of transcription 1 (STAT1) and STAT3 were characterized via
of NO, TNF-a, IL-1β and
IL-6 were meaningfully increased in β-glucan (polycan) treated RAW264.7
cells, along with the increased expression of inducible NO synthase, TNF-a, IL-6 and IL-1β mRNA at
concentrations with no cytotoxicity. β-glucan (polycan) treatment also
caused the NF-κB and phosphorylation of MAPKs, STAT1 and STAT3, showing
β-glucan (polycan) activated macrophages through activation of
NF-κB and MAPKs signaling pathways in RAW264.7 cells.
results reveal the therapeutic effects of β-glucan (polycan) may partly
be due to its ability to modulate immune functions in macrophages.
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