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Journal of Environmental Biology

pISSN: 0254-8704 ; eISSN: 2394-0379 ; CODEN: JEBIDP

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        Abstract - Issue Jan 2018, 39 (1)                                                                                                             Back



nstantaneous and historical temperature effects on a-pinene

Gene expression profiling and expression analysis of freshwater shrimp (Neocaridina denticulata denticulata) using expressed sequence tags and short-term exposure to copper

 

N. Y. Kim1, E. J. Jeon1, S. H. Jung1, S. J. Ahn2, M. A. Park3 and J. S. Seo3*

1Pathology Research Division, National Institute of Fisheries Science (NIFS), Busan-46083, Korea

2Fisheries R&D Management Center, Korea Institute of Marine Science & Technology Promotion (KIMST), Seoul-06775, Korea

3Aquatic Disease Control Division, National Institute of Fisheries Science (NIFS), Busan-46083, Korea

*Corresponding Author E-mail: pharm001@korea.kr

 

 

 

Key words

Biomarker

Copper

Expressed sequence tags

Neocaridina denticulata

Transcriptome

 

 

 

Publication Data

Paper received : 28.11.2015 Revised received : 23.05.2016 Re-revised received : 30.06.2016 Accepted : 05.07.2016

 

Abstract

Aim: The aim of the current study was to investigate environmental potential biomonitoring marker to exposure the copperfrom freshwater shrimp using expressed sequence tag (EST) analysis.

 

Methodology: The freshwater shrimp was collected from Lake Chang Pyung, South Korea and made cDNA libraries from whole body. To analyze expression patterns of putative biomarkersaccording to the individuals'exposure to copper in a time-and concentration-dependentmanners, Quantitative PCR (qPCR) was conducted.

 

Results: The expression levels of 9 potential biomarkers for heavy metal exposure including arginine kinase, acheron, cytochrome c oxidase subunit I, metallothionein, methylene tetrahydrofolatereductase, glutathione peroxidase, cathepsin L, cathepsin A and Cu/Zn superoxide dismutase genes were up-regulated 1-24 hr post-exposure to copper, in an exposure time- and/or dose-dependent manner. Furthermore, the discovery of genes that relate to heavy metal exposure and immune defense enables us to investigate the toxicity mechanisms and ecological effects of heavy metal pollution.

 

Interpretation: This study contributes to the identification of numerous EST clones that can be applied to further clarify the genetics and biomonitoring markers of freshwater shrimp.

 

 

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