of perylene and benzo [ghi] perylene (5-6 rings) using yeast
consortium : Kinetic study, enzyme analysis and degradation pathway
Mandal and N. Das*
Laboratory, School of Bio Sciences and Technology (SBST), VIT University,
Vellore - 632 014, India
Author E-mail: firstname.lastname@example.org
Paper received : 23.05.2016
Revised received : 29.11.2016
Re-revised received :
16.03.2017 Accepted : 08.08.2017
Aim: The present work
aims to demonstrate the ability of yeast consortium for degradation of
perylene and benzo[ghi]perylene which may contribute towards the
development of an appropriate in-situ bioremediation techniques.
of perylene (five ring) and benzo[ghi]perylene (six ring) was
evaluated using yeast consortia under optimized conditions. Rate of
biodegradation was tested with various kinetic models and intermediate
metabolites were identified by GC-MS and FTIR analysis. Involvement of
enzymes viz., 1,2-dioxygenase, 2,3-dioxygenase, catalase, laccase,
lignin peroxidase and manganese peroxide were noted during PAHs degradation.
yeast consortium YC02 (Hanseniaspora opuntiae NS02, Debaryomyces
hansenii NS03 and Hanseniaspora valbyensis NS04) degraded
67 % perylene at initial concentration 50 mg l-1, whereas YC04 (Rhodotorula
sp. NS01, Debaryomyces hansenii NS03 and Hanseniaspora valbyensis
NS04) degraded 60 % benzo[ghi]perylene at initial concentration 40 mg
l-1 after 6 days. The consortium YC02 and YC04 exhibited best reaction by
first order kinetics with half life period of 3.397 days and 4.218 days for
degradation of perylene and benzo[ghi]perylene, respectively.
on the metabolites identified by GC-MS and FTIR analysis, possible pathways
of perylene and benzo[ghi]perylene degradation were proposed. The
results of present study revealed that application of yeast consortium can be
a valuable approach towards remediation of high molecular weight PAHs having
more benzene rings.
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